CDCA7L Recombinant monoclonal antibody, PBS Only

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• CDCA7L Recombinant monoclonal antibody, PBS Only (83682-4-PBS)

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  WB analysis using 83682-4-RR (same clone as 83682-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83682-4-RR (CDCA7L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83682-4-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 83682-4-RR (same clone as 83682-4-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug CDCA7L Recombinant antibody (83682-4-RR, Clone:240639A3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83682-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83682-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83682-4-RR against Human CDCA7L were performed. The affinity constant is 7.75 nM.

CDCA7L Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 83682-4-PBS

Clone No.240639A3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

R1, Protein JPO2, JPO2, HR1, Cell division cycle-associated 7-like protein

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Product Information

83682-4-PBS targets CDCA7L in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag10944

  Product name: Recombinant human CDCA7L protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-269 aa of BC009352

  Sequence: MELATRYQIPKEVADIFNAPSDDEEFVGFRDDVPMETLSSEESCDSFDSLESGKQQDVRFHSKYFTEELRRIFIEDTDSETEDFAGFTQSDLNGKTNPEVMVVESDLSDDGKASLVSEEEEDEEEDKATPRRSRSRRSSIGLRVAFQFPTKKLANKPDKNSSSEQLFSSARLQNEKKTILERKKDCRQVIQREDSTSESEDDSRDESQESSDALLKRTMNIKENKAMLAQLLAELNSMPDFFPVRTPTSASRKKTVRRAFSEGQITRRM

• Full Name: cell division cycle associated 7-like
• Calculated Molecular Weight: 454 aa, 52 kDa
• GenBank Accession Number: BC009352
• Gene Symbol: CDCA7L
• Gene ID (NCBI): 55536
• RRID: AB_3671283
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96GN5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Cell division cycle-associated 7-like protein (CDCA7L) is also known as HR1 and JPO2 and belongs to the JPO protein family. CDCA7L is identified as a target gene of c-Myc and is frequently dysregulated in multiple cancers. CDCA7L can complement the c-Myc transformation-defective mutant W135E and potentiate Myc-mediated transformation (PMID: 24141559, PMID: 37140023). CDCA7L translocates from cytoplasm to nucleus under dexamethasone induction. CDCA7L is widely expressed. CDCA7L has 4 isoforms produced by alternative splicing (refer to UniProt).