CDCA8 Polyclonal antibody

• 

  WB analysis of mouse kidney using 12465-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12465-1-AP (CDCA8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12465-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12465-1-AP (CDCA8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 12465-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12465-1-AP (CDCA8 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 12465-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12465-1-AP (CDCA8 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of A431 using 12465-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CDCA8 antibody (12465-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 12465-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12465-1-AP (CDCA8 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

CDCA8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12465-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

BOR, Borealin, Cell division cycle-associated protein 8, Dasra B, Dasra-B

---

Tested Applications

• Positive WB detected in: A549 cells, mouse kidney tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Product Information

12465-1-AP targets CDCA8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3136

  Product name: Recombinant human CDCA8 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 1-280 aa of BC016944

  Sequence: MAPRKGSSRVANTNSLRRRKLASFLKDFDREVEIRIKQIESDRQNLLKEVDNLYNIEILRLPKALREMNWLDYFALGGNKQALEEAATADLDITEINKLTAEAIQTPLKSAKTRKVIQVDEMIVEEEEEEENERKNLQTARVKRCPPSKKRTQSIQGKGKGKRSSRANTVTPAVGRLEVSMVKPTPGLTPRFDSRVFKTPGLRTPAAGERIYNISGNGSPLADSKEIFLTVPVGGGESLRLLASDLQRHSIAQLDPEALGNIKKLSNRLAQICSSIRTHK

• Full Name: cell division cycle associated 8
• Calculated Molecular Weight: 280 aa, 31 kDa
• Observed Molecular Weight: 31-38 kDa
• GenBank Accession Number: BC016944
• Gene Symbol: CDCA8
• Gene ID (NCBI): 55143
• RRID: AB_2078783
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q53HL2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDCA8, also named as Borealin, Dasra-B and PESCRG3, is a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. This protein can be migrated to 35-40 kDa with phosphor modification or ~50 kDa with SUMO modification.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Science; ESCRT-III governs the Aurora B-mediated abscission checkpoint through CHMP4C.; Authors - Jeremy G Carlton
human	IHC	Front Pharmacol; A novel risk model of three SUMOylation genes based on RNA expression for potential prognosis and treatment sensitivity prediction in kidney cancer; Authors - Song-Chao Li
human	WB,IHC	J Cell Mol Med; Cell division cycle associated 8: A novel diagnostic and prognostic biomarker for hepatocellular carcinoma; Authors - Xiao-Han Cui
mouse	WB	Cell Mol Life Sci; Global phosphoproteomic analysis identified key kinases regulating male meiosis in mouse; Authors - Haojie Li
human	WB,IHC	Funct Integr Genomics; Let-7c-5p down-regulates immune-related CDCA8 to inhibit hepatocellular carcinoma; Authors - Wanjin Chen
human	WB,IHC	Am J Transl Res; Cell division cycle associated 8 promotes the growth and inhibits the apoptosis of endometrial cancer cells by regulating cell cycle and P53/Rb signaling pathway; Authors - Wenxue Li ; KD Validated