Tested Applications
| Positive WB detected in | A549 cells, mouse kidney tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12465-1-AP targets CDCA8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3136 Product name: Recombinant human CDCA8 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-280 aa of BC016944 Sequence: MAPRKGSSRVANTNSLRRRKLASFLKDFDREVEIRIKQIESDRQNLLKEVDNLYNIEILRLPKALREMNWLDYFALGGNKQALEEAATADLDITEINKLTAEAIQTPLKSAKTRKVIQVDEMIVEEEEEEENERKNLQTARVKRCPPSKKRTQSIQGKGKGKRSSRANTVTPAVGRLEVSMVKPTPGLTPRFDSRVFKTPGLRTPAAGERIYNISGNGSPLADSKEIFLTVPVGGGESLRLLASDLQRHSIAQLDPEALGNIKKLSNRLAQICSSIRTHK Predict reactive species |
| Full Name | cell division cycle associated 8 |
| Calculated Molecular Weight | 280 aa, 31 kDa |
| Observed Molecular Weight | 31-38 kDa |
| GenBank Accession Number | BC016944 |
| Gene Symbol | CDCA8 |
| Gene ID (NCBI) | 55143 |
| RRID | AB_2078783 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q53HL2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CDCA8, also named as Borealin, Dasra-B and PESCRG3, is a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. This protein can be migrated to 35-40 kDa with phosphor modification or ~50 kDa with SUMO modification.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CDCA8 antibody 12465-1-AP | Download protocol |
| IHC protocol for CDCA8 antibody 12465-1-AP | Download protocol |
| WB protocol for CDCA8 antibody 12465-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CDCA8 Polyclonal antibody (Cat# 12465-1-AP) has 13 citations across journals including Science, PLoS Biology, Cell & Molecular Life Sciences, Frontiers in Pharmacology, Journal of Cellular and Molecular Medicine, Functional & Integrative Genomics, Hepatobiliary & Pancreatic Diseases International, PLoS One, Discovery Oncology, World Journal of Gastrointestinal Oncology, Scientific Reports, Translational Pediatrics, and American Journal of Translational Research. Research fields span cell cycle regulation, cancer biology (hepatocellular, kidney, breast, Wilms, osteosarcoma, and endometrial cancers), phosphoproteomics, meiosis, and tumor prognosis.
| Species | Application | Title |
|---|---|---|
PLoS Biol A nonenzymatic dependency on inositol-requiring enzyme 1 controls cancer cell cycle progression and tumor growth | ||
Cell Mol Life Sci Global phosphoproteomic analysis identified key kinases regulating male meiosis in mouse | ||
Front Pharmacol A novel risk model of three SUMOylation genes based on RNA expression for potential prognosis and treatment sensitivity prediction in kidney cancer | ||
J Cell Mol Med Cell division cycle associated 8: A novel diagnostic and prognostic biomarker for hepatocellular carcinoma | ||
Funct Integr Genomics Let-7c-5p down-regulates immune-related CDCA8 to inhibit hepatocellular carcinoma |
