CDCA8 Monoclonal antibody, PBS Only

• 

  WB analysis using 68510-1-Ig (same clone as 68510-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68510-1-Ig (CDCA8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68510-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 68510-1-Ig (same clone as 68510-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CDCA8 antibody (68510-1-Ig, Clone: 2A10C9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68510-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 68510-1-Ig (same clone as 68510-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CDCA8 antibody (68510-1-Ig, Clone: 2A10C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68510-1-PBS in a different storage buffer formulation.

CDCA8 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 68510-1-PBS

Clone No.2A10C9

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

2A10C9, BOR, Borealin, Cell division cycle-associated protein 8, Dasra B

---

Product Information

68510-1-PBS targets CDCA8 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3136

  Product name: Recombinant human CDCA8 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 1-280 aa of BC016944

  Sequence: MAPRKGSSRVANTNSLRRRKLASFLKDFDREVEIRIKQIESDRQNLLKEVDNLYNIEILRLPKALREMNWLDYFALGGNKQALEEAATADLDITEINKLTAEAIQTPLKSAKTRKVIQVDEMIVEEEEEEENERKNLQTARVKRCPPSKKRTQSIQGKGKGKRSSRANTVTPAVGRLEVSMVKPTPGLTPRFDSRVFKTPGLRTPAAGERIYNISGNGSPLADSKEIFLTVPVGGGESLRLLASDLQRHSIAQLDPEALGNIKKLSNRLAQICSSIRTHK

• Full Name: cell division cycle associated 8
• Calculated Molecular Weight: 280 aa, 31 kDa
• Observed Molecular Weight: 31-38 kDa
• GenBank Accession Number: BC016944
• Gene Symbol: CDCA8
• Gene ID (NCBI): 55143
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q53HL2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CDCA8, also named as Borealin, Dasra-B and PESCRG3, is a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The predicted molecular weight of CDCA8 is about 31 kDa. This protein can be migrated to 35-40 kDa with phosphor modification or ~50 kDa with SUMO modification.