Tested Applications
| Positive WB detected in | MDA-MB-468 cells, A431 cells, HEK-293 cell, K-562 cells, Ramos cells, Daudi cells |
| Positive IF/ICC detected in | A549 cells, A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68510-1-Ig targets CDCA8 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3136 Product name: Recombinant human CDCA8 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-280 aa of BC016944 Sequence: MAPRKGSSRVANTNSLRRRKLASFLKDFDREVEIRIKQIESDRQNLLKEVDNLYNIEILRLPKALREMNWLDYFALGGNKQALEEAATADLDITEINKLTAEAIQTPLKSAKTRKVIQVDEMIVEEEEEEENERKNLQTARVKRCPPSKKRTQSIQGKGKGKRSSRANTVTPAVGRLEVSMVKPTPGLTPRFDSRVFKTPGLRTPAAGERIYNISGNGSPLADSKEIFLTVPVGGGESLRLLASDLQRHSIAQLDPEALGNIKKLSNRLAQICSSIRTHK Predict reactive species |
| Full Name | cell division cycle associated 8 |
| Calculated Molecular Weight | 280 aa, 31 kDa |
| Observed Molecular Weight | 31-38 kDa |
| GenBank Accession Number | BC016944 |
| Gene Symbol | CDCA8 |
| Gene ID (NCBI) | 55143 |
| RRID | AB_3085219 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q53HL2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CDCA8, also named as Borealin, Dasra-B and PESCRG3, is a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The predicted molecular weight of CDCA8 is about 31 kDa. This protein can be migrated to 35-40 kDa with phosphor modification or ~50 kDa with SUMO modification.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CDCA8 antibody 68510-1-Ig | Download protocol |
| WB protocol for CDCA8 antibody 68510-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
