CDK1/CDC2 Monoclonal antibody

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• CDK1/CDC2 Monoclonal antibody (67575-1-Ig)

CDK1/CDC2 Monoclonal Antibody for WB, FC (Intra), ELISA

Cat No. 67575-1-Ig Copied!

Clone No. 2A11E4

Publications (37)

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Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), CoIP, ELISA

Synonyms

CDC2, CDK1, 2A11E4, CDC28A, CDKN1

• 

  WB analysis using 67575-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67575-1-Ig (CDK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HeLa using 67575-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK1 Monoclonal antibody (67575-1-Ig, Clone:2A11E4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, A549 cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67575-1-Ig targets CDK1/CDC2 in WB, IHC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29018

  Product name: Recombinant human CDK1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-297 aa of BC014563

  Sequence: MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM

• Full Name: cell division cycle 2, G1 to S and G2 to M
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 30-34 kDa
• GenBank Accession Number: BC014563
• Gene Symbol: CDK1
• Gene ID (NCBI): 983
• RRID: AB_2882788
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P06493
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa.

Publications

What published studies show

The CDK1/CDC2 Monoclonal antibody (Cat# 67575-1-Ig) has 37 citations across journals including Acta Pharm Sin B, Cell Death & Disease, Phytomedicine, EMBO Reports, Stem Cell Research & Therapy, Cell Signaling, and iScience. Research fields span oncology (glioblastoma, breast, gastric, renal, prostate, ovarian, pancreatic, and colorectal cancers), cell cycle regulation, ferroptosis, neuroinflammation, vascular remodeling, DNA damage repair, and natural product–based drug discovery.

Automatically generated from the 37 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Acta Pharm Sin B; ADAR1 regulates vascular remodeling in hypoxic pulmonary hypertension through N1-methyladenosine modification of circCDK17; Authors - Junting Zhang
		Research (Wash D C); PRMT5-Mediated Arginine Methylation of ACSL4 Attenuates Its Stability and Suppresses Ferroptosis in Renal Cancer; Authors - Meng Zhang
human	WB,IHC,CoIP	Cell Death Dis; OTUD4 promotes the progression of glioblastoma by deubiquitinating CDK1 and activating MAPK signaling pathway; Authors - Mingxin Ci
human	WB	Cell Death Dis; The deubiquitinase YOD1 suppresses tumor progression by stabilizing ZNF24 in clear cell renal carcinoma; Authors - Ji Liu
human	WB	Phytomedicine; Kaempferol targets Src to exert its chemopreventive effects on mammary tumorigenesis via regulation of the PI3K/AKT pathway; Authors - Pinghan Wang
human, mouse	WB	Phytomedicine; Single-cell and bulk RNA-seq with machine learning reveals CCNB1/CDK1-mediated G2/M arrest as key anti-HCC mechanism of Fuzheng Xiaozheng Prescription.; Authors - Xia Li