Anti-Human CDK1 (POH-1)

• 

  WB analysis using 65182-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 65182-1-Ig (CDK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 65182-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 1 ug Anti-Human CDK1 (65182-1-Ig, Clone:POH-1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 1 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

CDK1 Monoclonal Antibody for WB, FC (Intra)

Cat No. 65182-1-Ig

Clone No.POH-1

Publications(3)

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

human, bovine and More (1)

Applications

WB, FC (Intra)

Synonyms

CDC2, CDC28A, CDKN1, Cell division protein kinase 1, Cyclin dependent kinase 1

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, HeLa cells, K-562 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 1 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

65182-1-Ig targets CDK1 in WB, FC (Intra) applications and shows reactivity with human, bovine samples.

• Tested Reactivity: human, bovine
• Cited Reactivity: human, sheep
• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  fusion protein

• Full Name: cell division cycle 2, G1 to S and G2 to M
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 30-34 kDa
• GenBank Accession Number: BC014563
• Gene Symbol: CDK1
• Gene ID (NCBI): 983
• RRID: AB_2918456
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Purified by protein-A affinity chromatography
• UNIPROT ID: P06493
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Discov Oncol; Unveiling the role of PSMA5 in glioma progression and prognosis; Authors - Wei Liu
sheep	WB	Zool Res; LncRNA GTL2 regulates myoblast proliferation and differentiation via the PKA-CREB pathway in Duolang sheep; Authors - Qian Chen
human	WB	Cancer Genomics Proteomics; Depletion of DNTTIP2 Induces Cell Cycle Arrest in Pancreatic Cancer Cells; Authors - Masato Yoshizawa