Anti-Human CDK1 (POH-1)

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  WB analysis using 65182-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 65182-1-Ig (CDK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 65182-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 1 ug Anti-Human CDK1 (65182-1-Ig, Clone:POH-1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 1 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

CDK1 Monoclonal Antibody for WB, FC (Intra)

Cat No. 65182-1-Ig

Clone No.POH-1

Publications(3)

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human, Bovine and More (2)

Applications

WB, FC (Intra)

Synonyms

CDC2, CDC28A, CDK1, Cell division protein kinase 1, Cyclin dependent kinase 1, DKFZp686L20222, p34 protein kinase

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, HeLa cells, K-562 cells
• Positive FC (Intra) detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 1 ug per 10^6 cells in 100 μl suspension
• Flow Cytometry (FC): FC :

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

65182-1-Ig targets CDK1 in WB, FC (Intra) applications and shows reactivity with Human, Bovine samples.

• Tested Reactivity: Human, Bovine
• Cited Reactivity: human, sheep
• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Bacterially expressed full-length human Cdk1 protein
• Full Name: cell division cycle 2, G1 to S and G2 to M
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 30-34 kDa
• GenBank Accession Number: BC014563
• Gene Symbol: CDK1
• Gene ID (NCBI): 983
• RRID: AB_2918456
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Purified by protein-A affinity chromatography
• UNIPROT ID: P06493
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Discov Oncol; Unveiling the role of PSMA5 in glioma progression and prognosis; Authors - Wei Liu
sheep	WB	Zool Res; LncRNA GTL2 regulates myoblast proliferation and differentiation via the PKA-CREB pathway in Duolang sheep; Authors - Qian Chen
human	WB	Cancer Genomics Proteomics; Depletion of DNTTIP2 Induces Cell Cycle Arrest in Pancreatic Cancer Cells; Authors - Masato Yoshizawa