CDK1 Monoclonal antibody

CDK1 Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), CoIP, ELISA

Conjugate

Unconjugated

Publications

13

CloneNo.

2A11E4

Cat no : 67575-1-Ig

Synonyms

CDC2, 2A11E4, Cyclin dependent kinase 1, Cell division protein kinase 1, CDKN1

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Validation Data Gallery

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  WB analysis using 67575-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67575-1-Ig (CDK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 67575-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK1 Monoclonal antibody (67575-1-Ig, Clone:2A11E4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HEK-293 cells, A549 cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 13 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

67575-1-Ig targets CDK1 in WB, IHC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CDK1 fusion protein Ag29018
• Full Name: cell division cycle 2, G1 to S and G2 to M
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC014563
• Gene Symbol: CDK1
• Gene ID (NCBI): 983
• RRID: AB_2882788
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa.

Publications

Species	Application	Title
mouse	WB	Acta Pharm Sin B; ADAR1 regulates vascular remodeling in hypoxic pulmonary hypertension through N1-methyladenosine modification of circCDK17; Authors - Junting Zhang
human	WB	Mol Carcinog; YAP/TEAD4-induced KIF4A contributes to the progression and worse prognosis of esophageal squamous cell carcinoma.; Authors - Ya Li
human	WB	Onco Targets Ther; Sinomenine Inhibits the Growth of Ovarian Cancer Cells Through the Suppression of Mitosis by Down-Regulating the Expression and the Activity of CDK1.; Authors - Xiaoyan Qu
human	WB	Chin Med; A network pharmacology approach to reveal the pharmacological targets and biological mechanism of compound kushen injection for treating pancreatic cancer based on WGCNA and in vitro experiment validation; Authors - Chao Wu
mouse	WB	BMC Cardiovasc Disord; Role of ferroptosis-related genes in coronary atherosclerosis and identification of key genes: integration of bioinformatics analysis and experimental validation; Authors - Qingwen Meng
human	WB	ACS Pharmacol Transl Sci; Identification of PDCD2 as a Candidate Target of Andrographolide That Arrests the Tumor Cell Cycle by Human Proteome-Scale Screening; Authors - Lei Wang