CDK5R1 Monoclonal antibody
CDK5R1 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse, rat, pig, rabbit
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1A3A5
Cat no : 67503-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse cerebellum tissue, mouse brain tissue, pig brain tissue, rat brain tissue |
Positive IHC detected in | mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
IHC | See 1 publications below |
Product Information
67503-1-Ig targets CDK5R1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.
Tested Reactivity | Human, mouse, rat, pig, rabbit |
Cited Reactivity | mouse |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | CDK5R1 fusion protein Ag13152 |
Full Name | cyclin-dependent kinase 5, regulatory subunit 1 (p35) |
Calculated molecular weight | 34 kDa |
Observed molecular weight | 34 kDa |
GenBank accession number | BC020580 |
Gene symbol | CDK5R1 |
Gene ID (NCBI) | 8851 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cyclin dependent kinase-5 (Cdk5), is an atypical but essential member of the Cdk family of proline-directed serine/threonine kinases. CDK5R1 encodes p35, a specific activator of the serine/threonine kinase CDK5, which plays a central role in neuronal migration and differentiation during central nervous system development. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form.
Protocols
Product Specific Protocols | |
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WB protocol for CDK5R1 antibody 67503-1-Ig | Download protocol |
IHC protocol for CDK5R1 antibody 67503-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Ther Nucleic Acids Implications of miR-148a-3p/p35/PTEN signaling in tau hyperphosphorylation and autoregulatory feedforward of Akt/CREB in Alzheimer's disease. |