• Featured Product
  • KD/KO Validated

CDK6 Monoclonal antibody

CDK6 Monoclonal Antibody for IF, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF,ELISA

Conjugate

Unconjugated

CloneNo.

4B9C11

Cat no : 66278-1-Ig

Synonyms

CDK6, Cell division protein kinase 6, cyclin dependent kinase 6, PLSTIRE



Tested Applications

Positive WB detected inHEK-293 cells, THP-1 cells, Jurkat cells, NIH/3T3 cells, HeLa cells, ROS1728 cells, C6 cells, Raw 264.7 cells
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66278-1-Ig targets CDK6 in WB, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CDK6 fusion protein Ag5600
Full Name cyclin-dependent kinase 6
Calculated molecular weight 326 aa, 36 kDa
Observed molecular weight 36-40 kDa
GenBank accession numberBC027989
Gene symbol CDK6
Gene ID (NCBI) 1021
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDK6(Cyclin-dependent kinase 6) is involved in initiation and maintenance of cell cycle exit during cell differentiation and it prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types.The molecular weight of CDK6 is 36-40 KDa (PMID: 23563707, 8114739). 

Protocols

Product Specific Protocols
WB protocol for CDK6 antibody 66278-1-IgDownload protocol
IF protocol for CDK6 antibody 66278-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Signal

circ_ARF3 regulates the pathogenesis of osteosarcoma by sponging miR-1299 to maintain CDK6 expression.

Authors - Ai-Mei Gao