CDK6 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CDK6 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4B9C11

Cat no : 66278-1-PBS

Synonyms

CDK6, Cell division protein kinase 6, cyclin dependent kinase 6, PLSTIRE

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Validation Data Gallery

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  WB analysis using 66278-1-Ig (same clone as 66278-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66278-1-Ig (CDK6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66278-1-Ig (same clone as 66278-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66278-1-Ig (CDK6 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66278-1-Ig (same clone as 66278-1-PBS)

  WB result of CDK6 antibody (66278-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK6 transfected HEK-293 cells. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66278-1-Ig (same clone as 66278-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66278-1-Ig (CDK6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 66278-1-Ig (same clone as 66278-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66278-1-Ig (CDK6 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66278-1-Ig (same clone as 66278-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66278-1-Ig (CDK6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

• 

  WB analysis of THP-1 using 66278-1-Ig (same clone as 66278-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66278-1-Ig (CDK6 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66278-1-Ig (same clone as 66278-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66278-1-Ig (CDK6 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66278-1-Ig (same clone as 66278-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66278-1-Ig(CDK6 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66278-1-PBS targets CDK6 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CDK6 fusion protein Ag5600
• Full Name: cyclin-dependent kinase 6
• Calculated Molecular Weight: 326 aa, 36 kDa
• Observed Molecular Weight: 36-40 kDa
• GenBank Accession Number: BC027989
• Gene Symbol: CDK6
• Gene ID (NCBI): 1021
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment.

Background Information

CDK6(Cyclin-dependent kinase 6) is involved in initiation and maintenance of cell cycle exit during cell differentiation and it prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types.The molecular weight of CDK6 is 36-40 KDa (PMID: 23563707, 8114739).