CEA Monoclonal antibody

CEA Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B8H6

Cat no : 68377-1-Ig

Synonyms

Carcinoembryonic antigen, CD66e, CEA, CEACAM5, DKFZp781M2392, Meconium antigen 100

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Validation Data Gallery

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  WB analysis using 68377-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68377-1-Ig (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68377-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68377-1-Ig (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 68377-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68377-1-Ig (CEACAM5 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 68377-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68377-1-Ig (CEACAM5 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 68377-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68377-1-Ig (CEA antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 68377-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68377-1-Ig (CEA antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HaCaT cells, human saliva, HT-29 cells, human rectum tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:2500-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68377-1-Ig targets CEA in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: carcinoembryonic antigen-related cell adhesion molecule 5
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 180-200 kDa
• GenBank Accession Number: BC034671
• Gene Symbol: CEACAM5
• Gene ID (NCBI): 1048
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis.