Filter: AllWBIHC

WB analysis using 68377-1-Ig (same clone as 68377-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68377-1-Ig (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.
WB analysis using 68377-1-Ig (same clone as 68377-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68377-1-Ig (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 68377-1-Ig (same clone as 68377-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68377-1-Ig (CEACAM5 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 68377-1-Ig (same clone as 68377-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68377-1-Ig (CEACAM5 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 68377-1-Ig (same clone as 68377-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68377-1-Ig (CEA antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 68377-1-Ig (same clone as 68377-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68377-1-Ig (CEA antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 68377-1-Ig (same clone as 68377-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68377-1-Ig (CEA antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 68377-1-Ig (same clone as 68377-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68377-1-Ig (CEA antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 68377-1-Ig (same clone as 68377-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 68377-1-Ig (CEA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 68377-1-Ig (same clone as 68377-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 68377-1-Ig (CEA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

CEA/CD66e Monoclonal antibody, PBS Only

CEA/CD66e Monoclonal Antibody for WB, IHC, ELISA

Cat No. 68377-1-PBS

Clone No.2B8H6

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CEA, 2B8H6, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, CD66e

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

68377-1-PBS targets CEA/CD66e in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Eg0247 Product name: Recombinant Human CEACAM5 protein (hIGg2fc Tag) Source: mammalian cells-derived, pINFUSE-IGSF8-CFc2(A2) Tag: C-hIGg2fc Domain: 35-685 aa of BC034671 Sequence: KLTIESTPFNVAEGKEVLLLVHNLPQHLFGYSWYKGERVDGNRQIIGYVIGTQQATPGPAYSGREIIYPNASLLIQNIIQNDTGFYTLHVIKSDLVNEEATGQFRVYPELPKPSISSNNSKPVEDKDAVAFTCEPETQDATYLWWVNNQSLPVSPRLQLSNGNRTLTLFNVTRNDSASYKCETQNPVSARRSDSVILNVLYGPDAPTISPLNTSYRSGENLNLSCHAASNPPAQYSWFVNGTFQQSTQELFIPNITVNNSGSYTCQAHNSDTGLNRTTVTTITVYAEPPKPFITSNNSNPVEDEDAVALTCEPEIQNTTYLWWVNNQSLPVSPRLQLSNDNRTLTLLSVTRNDVGPYECGIQNELSVDHSDPVILNVLYGPDDPTISPSYTYYRPGVNLSLSCHAASNPPAQYSWLIDGNIQQHTQELFISNITEKNSGLYTCQANNSASGHSRTTVKTITVSAELPKPSISSNNSKPVEDKDAVAFTCEPEAQNTTYLWWVNGQSLPVSPRLQLSNGNRTLTLFNVTRNDARAYVCGIQNSVSANRSDPVTLDVLYGPDTPIISPPDSSYLSGANLNLSCHSASNPSPQYSWRINGIPQQHTQVLFIAKITPNNNGTYACFVSNLATGRNNSIVKSITVSASGTSPGLSA Predict reactive species
Full Name	carcinoembryonic antigen-related cell adhesion molecule 5
Calculated Molecular Weight	77 kDa
Observed Molecular Weight	180-200 kDa
GenBank Accession Number	BC034671
Gene Symbol	CEA
Gene ID (NCBI)	1048
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P06731
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis.