Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, MDA-MB-231 cells, SK-BR-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
Product Information
23895-1-AP targets CEBPD in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20953 Product name: Recombinant human CEBPD protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-193 aa of BC105109 Sequence: MSAALFSLDGPARGAPWPAEPAPFYEPGRAGKPGRGAEPGALGEPGAAAPAMYDDESAIDFSAYIDSMAAVPTLELCHDELFADLFNSNHKAGGAGPLELLPGGPARPLGPGPAAPRLLKREPDWGDGDAPGSLLPAQVAACAQTVVSLAAAGQPTPPTSPEPPRSSPRQTPAPGPAREKSAGKRGPDRGSPE Predict reactive species |
| Full Name | CCAAT/enhancer binding protein (C/EBP), delta |
| Calculated Molecular Weight | 269 aa, 28 kDa |
| Observed Molecular Weight | 28-36 kDa |
| GenBank Accession Number | BC105109 |
| Gene Symbol | CEBPD |
| Gene ID (NCBI) | 1052 |
| RRID | AB_3742095 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | P49716 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CEBPD, a versatile transcription factor, belongs to the C/EBP family that comprises three parts: an N-terminal transactivating region, a basic DNA binding region, and a C-terminal leucine zipper domain (PMID: 17170124). It presents as a regulator in terms of cell differentiation, proliferation, motility, growth arrest, and cell death (PMID: 24155666). The apparent molecular weight is larger than the calculated molecular weight of 28 kDa, which is likely due to posttranslational modification, presumably by ubiquitination.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CEBPD antibody 23895-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
