CEL Polyclonal antibody

• Featured Product
• KD/KO Validated

CEL Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 15384-1-AP

Synonyms

BAL, Bile salt stimulated lipase, Bile salt-activated lipase, BSDL, BSSL, Bucelipase, Carboxyl ester lipase, CEase, CEL, CELL, Cholesterol esterase, FAP, FAPP, LIPA, MODY8, Pancreatic lysophospholipase, Sterol esterase

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Validation Data Gallery

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  WB analysis of human liver using 15384-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15384-1-AP (CEL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 15384-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15384-1-AP (CEL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15384-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15384-1-AP (CEL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15384-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15384-1-AP (CEL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 15384-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15384-1-AP (CEL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 15384-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15384-1-AP (CEL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human pancreas cancer using 15384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using CEL antibody (15384-1-AP) at dilution of 1:200 and TRITC-Goat anti-Rabbit IgG, CoraLite® Plus 647 Glucagon antibody (CL647-15954, Magenta), CoraLite®488 INS antibody (CL488-66198, Clone: 4B6A7, green).

• 

  IF Staining of human pancreas cancer using 15384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using CEL antibody (15384-1-AP) at dilution of 1:200 and TRITC-Goat anti-Rabbit IgG, CoraLite® Plus 647 Glucagon antibody (CL647-15954, Magenta), CoraLite®488 INS antibody (CL488-66198, Clone: 4B6A7, green).

• 

  IF Staining of HeLa using 15384-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15384-1-AP (CEL antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human liver tissue, HeLa cells, mouse heart tissue, mouse liver tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human pancreas cancer tissue, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

15384-1-AP targets CEL in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEL fusion protein Ag5262
• Full Name: carboxyl ester lipase (bile salt-stimulated lipase)
• Calculated Molecular Weight: 79 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC042510
• Gene Symbol: CEL
• Gene ID (NCBI): 1056
• RRID: AB_2229297
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Bile salt-activated lipase, previously named cholesterol esterase or bile salt-stimulated (or dependent) lipase, is a 74 kDa lipolytic enzyme capable of hydrolyzing cholesteryl esters, tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide(PMID:12454261). The same enzyme is present as a major protein in milk and as a minor constituent in liver, activated macrophages, and endothelial cells(PMID:11733511). Defects in CEL are a cause of maturity-onset diabetes of the young type 8 with exocrine dysfunction (MODY8)(PMID:16369531). The full length protein has 11 glycosylation sites. It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	IF	J Crohns Colitis; Exosomal miR-103a-3p from Crohn's creeping fat-derived ASCs contributes to intestinal fibrosis by targeting TGFBR3 and activating fibroblasts; Authors - Wenwei Qian
mouse	WB,IHC	Cell Mol Gastroenterol Hepatol; The CEL-HYB1 Hybrid Allele Promotes Digestive Enzyme Misfolding and Pancreatitis in Mice.; Authors - Xiao-Tong Mao
human	WB,IF	ACS Biomater Sci Eng; LINC01915 Facilitates the Conversion of Normal Fibroblasts into Cancer-Associated Fibroblasts Induced by Colorectal Cancer-Derived Extracellular Vesicles through the miR-92a-3p/KLF4/CH25H Axis.; Authors - Minghe Zhou
HUMAN	WB	Biochemistry; Branched Fatty Acid Esters of Hydroxy Fatty Acids Are Preferred Substrates of the MODY8 Protein Carboxyl Ester Lipase.; Authors - Matthew J Kolar ; KO Validated
human	IF,IHC,WB	Heliyon; Integrative analysis of cancer-associated fibroblast signature in gastric cancer; Authors - Zidan Zhao