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CEL Polyclonal antibody, PBS Only (15384-1-PBS)

Filter: AllWBIHCIF-PIF/ICC

WB analysis of human liver using 15384-1-AP (same clone as 15384-1-PBS)
human liver tissue were subjected to SDS PAGE followed by western blot with 15384-1-AP (CEL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.
WB analysis of mouse heart using 15384-1-AP (same clone as 15384-1-PBS)
mouse heart tissue were subjected to SDS PAGE followed by western blot with 15384-1-AP (CEL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.
WB analysis of mouse liver using 15384-1-AP (same clone as 15384-1-PBS)
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15384-1-AP (CEL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 15384-1-AP (same clone as 15384-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 15384-1-AP (CEL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 15384-1-AP (same clone as 15384-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15384-1-AP (CEL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 15384-1-AP (same clone as 15384-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15384-1-AP (CEL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.
IF Staining of human pancreas cancer using 15384-1-AP (same clone as 15384-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using CEL antibody (15384-1-AP) at dilution of 1:200 and TRITC-Goat anti-Rabbit IgG, CoraLite® Plus 647 Glucagon antibody (CL647-15954, Magenta), CoraLite®488 INS antibody (CL488-66198, Clone: 4B6A7, green). This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.
IF Staining of human pancreas cancer using 15384-1-AP (same clone as 15384-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using CEL antibody (15384-1-AP) at dilution of 1:200 and TRITC-Goat anti-Rabbit IgG, CoraLite® Plus 647 Glucagon antibody (CL647-15954, Magenta), CoraLite®488 INS antibody (CL488-66198, Clone: 4B6A7, green). This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.
IF Staining of human pancreas cancer using 15384-1-AP (same clone as 15384-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using CEL antibody (15384-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), INS antibody (66198-1-Ig, Clone: 4B6A7, red). This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 15384-1-AP (same clone as 15384-1-PBS)
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15384-1-AP (CEL antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

CEL Polyclonal antibody, PBS Only

Name: CEL Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 15384-1-PBS

CEL Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 15384-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

15384-1-PBS targets CEL in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5262 Product name: Recombinant human CEL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 371-722 aa of BC042510 Sequence: SEFTITKGLRGAKTTFDVYTESWAQDPSQENKKKTVVDFETDVLFLVPTEIALAQHRANAKSAKTYAYLFSHPSRMPVYPKWVGADHADDIQYVFGKPFATPTGYRPQDRTVSKAMIAYWTNFAKTGDPNMGDSAVPTHWEPYTTENSGYLEITKKMGSSSMKRSLRTNFLRYWTLTYLALPTVTDQEATPVPPTGDSEATPVPPTGDSETAPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDAGPPPVPPTGDSGAPPVPPTGDSGAPPVTPTGDSETAPVPPTGDSGAPPVPPTGDSEAAPVPPTDDSKEAQMPAVIRF Predict reactive species
Full Name	carboxyl ester lipase (bile salt-stimulated lipase)
Calculated Molecular Weight	79 kDa
Observed Molecular Weight	67 kDa
GenBank Accession Number	BC042510
Gene Symbol	CEL
Gene ID (NCBI)	1056
RRID	AB_2229297
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P19835
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Bile salt-activated lipase, previously named cholesterol esterase or bile salt-stimulated (or dependent) lipase, is a 74 kDa lipolytic enzyme capable of hydrolyzing cholesteryl esters, tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide(PMID:12454261). The same enzyme is present as a major protein in milk and as a minor constituent in liver, activated macrophages, and endothelial cells(PMID:11733511). Defects in CEL are a cause of maturity-onset diabetes of the young type 8 with exocrine dysfunction (MODY8)(PMID:16369531). The full length protein has 11 glycosylation sites. It has 2 isoforms produced by alternative splicing.