Tested Applications
| Positive IF/ICC detected in | SH-SY5Y cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83115-1-RR targets CELSR2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | cadherin, EGF LAG seven-pass G-type receptor 2 (flamingo homolog, Drosophila) |
| Calculated Molecular Weight | 318 kDa |
| GenBank Accession Number | NM_001408 |
| Gene Symbol | CELSR2 |
| Gene ID (NCBI) | 1952 |
| RRID | AB_3670827 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q9HCU4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CELSR2, also named CDHF10, EGFL2, KIAA0279, and MEGF3, belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. It is a receptor that may be important in cell/cell signaling during nervous system formation.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CELSR2 antibody 83115-1-RR | Download protocol |
| IF protocol for CELSR2 antibody 83115-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The product has one review (5/5 stars) from a researcher who used it for Western Blot at a 1:1000 dilution. The reviewer reported positive results, stating it performed well for their WB application. Overall, the limited feedback is favorable, with the user confirming reliable performance under standard Western Blot conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
MALLIKARJUNA (Verified Customer) (10-24-2025) | GOOD FOR WESTERN BLOT
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