CENPC Polyclonal antibody

CENPC Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14250-1-AP

Synonyms

CENP C, CENP C 1, CENPC, CENPC1, Centromere autoantigen C, centromere protein C 1, hcp 4, ICEN7, MIF2

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Validation Data Gallery

• 

  WB analysis of HeLa using 14250-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14250-1-AP (CENPC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14250-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14250-1-AP (CENPC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 14250-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14250-1-AP (CENPC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14250-1-AP targets CENPC in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CENPC fusion protein Ag5523
• Full Name: centromere protein C 1
• Calculated Molecular Weight: 107 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC041117
• Gene Symbol: CENPC
• Gene ID (NCBI): 1060
• RRID: AB_10642008
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.