CENPN Polyclonal antibody

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CENPN Polyclonal Antibody for WB, ELISA

Cat No. 16751-1-AP Copied!

Publications (4)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

BM039, C16orf60, CENP N, CENPN, centromere protein N, ICEN32

• 

  WB analysis of MCF-7 using 16751-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16751-1-AP (CENPN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16751-1-AP targets CENPN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10219

  Product name: Recombinant human CENPN protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-331 aa of BC008972

  Sequence: MDETVAEFIKRTILKIPMNELTTILKAWDFLSENQLQTVNFRQRKESVVQHLIHLCEEKRASISDAALLDIIYMQFHQHQKVWDVFQMSKGPGEDVDLFDMKQFKNSFKKILQRALKNVTVSFRETEENAVWIRIAWGTQYTKPNQYKPTYVVYYSQTPYAFTSSSMLRRNTPLLGQALTIASKHHQIVKMDLRSRYLDSLKAIVFKQYNQTFETHNSTTPLQERSLGLDINMDSRIIHENIVEKERVQRITQETFGDYPQPQLEFAQYKLETKFKSGLNGSILAEREEPLRCLIKFSSPHLLEALKSLAPAGIADAPLSPLLTCIPNKRM

• Full Name: centromere protein N
• Calculated Molecular Weight: 339 aa, 40 kDa
• Observed Molecular Weight: 40 kDa, 25-30 kDa
• GenBank Accession Number: BC008972
• Gene Symbol: CENPN
• Gene ID (NCBI): 55839
• RRID: AB_2878310
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96H22
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CENPN, also named as C16orf60 and ICEN32, is a component of the CENPA-NAC complex. It is required for chromosome congression and efficiently align the chromosomes on a metaphase plate. CENPN has 4 isoforms with MW 24 kDa and 35-41 kDa.

Publications

What published studies show

The anti-CENPN antibody (Cat# 16751-1-AP) has 4 total citations published in J Cancer, Cancer Reports, Hormones and Metabolic Research, and Translational Cancer Research. These studies span cancer biology, investigating CENPN's roles in oral cancer proliferation, gastric adenocarcinoma biomarkers, esophageal cancer glucose metabolism via PI3K/AKT signaling, and pan-cancer diagnostics with breast cancer validation. Applications include WB and IHC in human samples.

Automatically generated from the 4 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	J Cancer; Centromere Protein N Participates in Cellular Proliferation of Human Oral Cancer by Cell-Cycle Enhancement.; Authors - Noritoshi Oka ; KD Validated
human	WB,IHC	Cancer Rep (Hoboken); High expression of centromere protein N as novel biomarkers for gastric adenocarcinoma; Authors - Xiaojie Wang ; KD Validated
human	WB	Horm Metab Res; Knockdown of CENPN Inhibits Glucose Metabolism and Induces G1 Arrest in Esophageal Cancer Cells by Regulating PI3K/AKT Signaling Pathway; Authors - Shasha Xu
human	WB	Transl Cancer Res; Diagnostics and immunological function of CENPN in human tumors: from pan-cancer analysis to validation in breast cancer; Authors - Yubo Jing