CENPN Polyclonal antibody

• 

  WB analysis of MCF-7 using 16751-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16751-1-AP (CENPN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

CENPN Polyclonal Antibody for WB, ELISA

Cat No. 16751-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

BM039, C16orf60, CENP N, CENPN, centromere protein N, ICEN32

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Tested Applications

• Positive WB detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Product Information

16751-1-AP targets CENPN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10219

  Product name: Recombinant human CENPN protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-331 aa of BC008972

  Sequence: MDETVAEFIKRTILKIPMNELTTILKAWDFLSENQLQTVNFRQRKESVVQHLIHLCEEKRASISDAALLDIIYMQFHQHQKVWDVFQMSKGPGEDVDLFDMKQFKNSFKKILQRALKNVTVSFRETEENAVWIRIAWGTQYTKPNQYKPTYVVYYSQTPYAFTSSSMLRRNTPLLGQALTIASKHHQIVKMDLRSRYLDSLKAIVFKQYNQTFETHNSTTPLQERSLGLDINMDSRIIHENIVEKERVQRITQETFGDYPQPQLEFAQYKLETKFKSGLNGSILAEREEPLRCLIKFSSPHLLEALKSLAPAGIADAPLSPLLTCIPNKRM

• Full Name: centromere protein N
• Calculated Molecular Weight: 339 aa, 40 kDa
• Observed Molecular Weight: 40 kDa, 25-30 kDa
• GenBank Accession Number: BC008972
• Gene Symbol: CENPN
• Gene ID (NCBI): 55839
• RRID: AB_2878310
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96H22
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CENPN, also named as C16orf60 and ICEN32, is a component of the CENPA-NAC complex. It is required for chromosome congression and efficiently align the chromosomes on a metaphase plate. CENPN has 4 isoforms with MW 24 kDa and 35-41 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	J Cancer; Centromere Protein N Participates in Cellular Proliferation of Human Oral Cancer by Cell-Cycle Enhancement.; Authors - Noritoshi Oka ; KD Validated
human	WB,IHC	Cancer Rep (Hoboken); High expression of centromere protein N as novel biomarkers for gastric adenocarcinoma; Authors - Xiaojie Wang ; KD Validated
human	WB	Horm Metab Res; Knockdown of CENPN Inhibits Glucose Metabolism and Induces G1 Arrest in Esophageal Cancer Cells by Regulating PI3K/AKT Signaling Pathway; Authors - Shasha Xu
human	WB	Transl Cancer Res; Diagnostics and immunological function of CENPN in human tumors: from pan-cancer analysis to validation in breast cancer; Authors - Yubo Jing