CEP120 Polyclonal antibody

CEP120 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24067-1-AP

Synonyms

CCDC100, centrosomal protein 120kDa, Centrosomal protein of 120 kDa, CEP120

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Validation Data Gallery

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  WB analysis of mouse lung using 24067-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 24067-1-AP (CEP120 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24067-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24067-1-AP (CEP120 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 24067-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24067-1-AP (CEP120 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 24067-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24067-1-AP (CEP120 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 24067-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP120 antibody (24067-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

• 

  IF Staining of HeLa using 24067-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP120 antibody (24067-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse lung tissue
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24067-1-AP targets CEP120 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEP120 fusion protein Ag21165
• Full Name: centrosomal protein 120kDa
• Calculated Molecular Weight: 986 aa, 113 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC040527
• Gene Symbol: CEP120
• Gene ID (NCBI): 153241
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP120 encodes a protein that functions in the microtubule-dependent coupling of the nucleus and the centrosome. It is involved in regulating centrosome-mediated interkinetic nuclear migration of neural progenitors (PubMed: 27185865, PubMed: 23857771). CEP120, SPICE1 and CPAP are required for centriole elongation and for recruitment of distal microtubule-capping proteins and CEP135 to procentrioles (PubMed: 23810536). Cep120 and Taccs are required for the neural progenitor pool during mouse neocortical development (PubMed: 17920017).