CEP128 Polyclonal antibody

CEP128 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 24810-1-AP

Synonyms

C14orf145, C14orf61, CEP128

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Validation Data Gallery

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  WB analysis of HEK-293T using 24810-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 24810-1-AP (CEP128 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 24810-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CEP128 antibody (24810-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 24810-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP128 antibody (24810-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CEP63 antibody (66996-1-Ig, Clone: 2G12B2, red).

• 

  IF Staining of HeLa using 24810-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP128 antibody (24810-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CEP63 antibody (66996-1-Ig, Clone: 2G12B2, red).

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Tested Applications

• Positive WB detected in: HEK-293T cells
• Positive IF detected in: HeLa cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24810-1-AP targets CEP128 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEP128 fusion protein Ag20610
• Full Name: chromosome 14 open reading frame 145
• Calculated Molecular Weight: 1094 aa, 128 kDa
• Observed Molecular Weight: 128 kDa
• GenBank Accession Number: BC045834
• Gene Symbol: C14orf145
• Gene ID (NCBI): 145508
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Centrosomal protein of 128kDa is encoded by CEP128 gene in humans. CEP128 localizes to the subdistal appendages of the mother centriole and regulates TGF-β/BMP signaling at the primary cilium (PMID: 29514088). Centrosomal protein CEP128 plays a role in male fertility and its variants affected the ciliogenesis in testes (PMID: 35296684).