CEP162 Polyclonal antibody

CEP162 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30210-1-AP

Synonyms

C6orf84, CEP162, FLJ13551, KIAA1009, Protein QN1 homolog, QN1

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Validation Data Gallery

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  WB analysis using 30210-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30210-1-AP (CEP162 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 30210-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30210-1-AP (CEP162 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 30210-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30210-1-AP (CEP162 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 30210-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30210-1-AP (CEP162 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 30210-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30210-1-AP (CEP162 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IHC detected in: human liver tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30210-1-AP targets CEP162 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEP162 fusion protein Ag32953
• Full Name: KIAA1009
• Calculated Molecular Weight: 162 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: NM_001286206.1
• Gene Symbol: KIAA1009
• Gene ID (NCBI): 22832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP162, also known as KIAA1009 and QN1, was found in organisms ranging from trypanosomes to vertebrates. A role for CEP162, involved in required to promote assembly of the transition zone in primary cilia. CEP162 acts by specifically recognizing and binding the axonemal microtubule. CEP162 localizes to the distal ends of centrioles before ciliogenesis and directly binds to axonemal microtubule, thereby promoting and restricting transition zone formation specifically at the cilia base.