CEP162 Polyclonal antibody, PBS Only

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• CEP162 Polyclonal antibody, PBS Only (30210-1-PBS)

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  WB analysis using 30210-1-AP (same clone as 30210-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30210-1-AP (CEP162 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30210-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 30210-1-AP (same clone as 30210-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30210-1-AP (CEP162 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30210-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 30210-1-AP (same clone as 30210-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30210-1-AP (CEP162 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30210-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 30210-1-AP (same clone as 30210-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30210-1-AP (CEP162 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30210-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 30210-1-AP (same clone as 30210-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30210-1-AP (CEP162 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30210-1-PBS in a different storage buffer formulation.

CEP162 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 30210-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

30210-1-PBS targets CEP162 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag32953

  Product name: Recombinant human CEP162 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 241-438 aa of NM_001286206.1

  Sequence: VLLDSLDSVAEVNLDEQDKITPKPRCLPEMTENEMTGTGVSYGQSSSDVEALHQAYCHIAHSLGDEDKQKIESNTVEDIKSSVKGHPQENEENSKNISTMESDLPTVEELMKPIRIDSFGISGFDLQPVSSEKVAERKETEFFSSLPLKMNPNILSQDSQHVNLFFDKNDENVILQKTTNESMENSCPQVTEVTATEE

• Full Name: KIAA1009
• Calculated Molecular Weight: 162 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: NM_001286206.1
• Gene Symbol: CEP162
• Gene ID (NCBI): 22832
• RRID: AB_2935531
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5TB80
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CEP162, also known as KIAA1009 and QN1, was found in organisms ranging from trypanosomes to vertebrates. A role for CEP162, involved in required to promote assembly of the transition zone in primary cilia. CEP162 acts by specifically recognizing and binding the axonemal microtubule. CEP162 localizes to the distal ends of centrioles before ciliogenesis and directly binds to axonemal microtubule, thereby promoting and restricting transition zone formation specifically at the cilia base.