CEP170/CEP170L Polyclonal antibody

• Featured Product
• KD/KO Validated

CEP170/CEP170L Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 18899-1-AP

Synonyms

KIAA0470, KIAA0470L, CEP170L, CEP170

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Validation Data Gallery

• 

  WB analysis using 18899-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18899-1-AP (CEP170/CEP170L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 18899-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18899-1-AP (CEP170/CEP170L antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 18899-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 18899-1-AP (CEP170/CEP170L antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 18899-1-AP

  Immunofluorescent analysis of Hela cells, using CEP170L antibody 18899-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse testis tissue, rat testis tissue
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 3 publications below

Product Information

18899-1-AP targets CEP170/CEP170L in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEP170/CEP170L fusion protein Ag13532
• Full Name: centrosomal protein 170kDa-like
• Calculated Molecular Weight: 170 kDa
• Observed Molecular Weight: 170-200 kDa
• GenBank Accession Number: BC014590
• Gene Symbol: CEP170
• Gene ID (NCBI): 645455
• RRID: AB_2878566
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP170, also named as FAM68A, KAB and KIAA0470, is a fork head-associated (FHA) domain protein. CEP170L is a part of CEP170. The gene CEP170 has some isoforms with MW 170-200 kDa, 160 kDa and some fragments 30-40 kDa, ~75 kDa.

Publications

Species	Application	Title
human	WB,IF,IP	Mol Cancer; CHEK1 and circCHEK1_246aa evoke chromosomal instability and induce bone lesion formation in multiple myeloma.; Authors - Chunyan Gu
human	WB,IP	Signal Transduct Target Ther; BUB1B and circBUB1B_544aa aggravate multiple myeloma malignancy through evoking chromosomal instability.; Authors - Xiaozhu Tang ; KD Validated
human	WB	Acta Pharm Sin B; NAT10 promotes cell proliferation by acetylating CEP170 mRNA to enhance translation efficiency in multiple myeloma; Authors - Rongfang Wei
human	WB,IP	Cell Insight; LUC7L3 is a downstream factor of SRSF1 and prevents genomic instability; Authors - Xiaqing Zhang