CEP170/CEP170L Polyclonal antibody

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• CEP170/CEP170L Polyclonal antibody (18899-1-AP)

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• KD/KO Validated

CEP170/CEP170L Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 18899-1-AP Copied!

Publications (5)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA
For FC, select conjugated version.

Synonyms

KIAA0470, CEP170, CEP170L, KIAA0470L

• 

  WB analysis using 18899-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18899-1-AP (CEP170/CEP170L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse testis using 18899-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18899-1-AP (CEP170/CEP170L antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat testis using 18899-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 18899-1-AP (CEP170/CEP170L antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 18899-1-AP

  IP result of anti-CEP170/CEP170L (IP:18899-1-AP, 4ug; Detection:18899-1-AP 1:4000) with HeLa cells lysate 1480 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 18899-1-AP

  Immunofluorescent analysis of Hela cells, using CEP170L antibody 18899-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, mouse testis tissue, rat testis tissue
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18899-1-AP targets CEP170/CEP170L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13532

  Product name: Recombinant human CEP170L protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-293 aa of BC014590

  Sequence: MPTSSSFKHRIKEQEDYIRDWTAHREEIARISQDLALIAREINDVAGEIDSVTSSGTAPSTTLVDRVFDESLNFQKIPPLVHSKTPEGNNGRSGDPRPQAAEPPDHLTITRRRTWSRDEVMGDNLLLSSVFQFSKKIRQSIDKTAGKIRILFKDKDRNWDDIESKLRAESEVPIVKTSSMEISSILQELKRVEKQLQAINAMIDPDGTLEALNNMGFPSAMLPSPPKQKSSPVNNHHSPGQTPTLGQPEARALHPAAVSAAAEFENAESEADFSIHFNRVNPDGEEEDVTVHK

• Full Name: centrosomal protein 170kDa-like
• Calculated Molecular Weight: 170 kDa
• Observed Molecular Weight: 170-200 kDa
• GenBank Accession Number: BC014590
• Gene Symbol: CEP170
• Gene ID (NCBI): 645455
• RRID: AB_2878566
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96L14
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP170, also named as FAM68A, KAB and KIAA0470, is a fork head-associated (FHA) domain protein. CEP170L is a part of CEP170. The gene CEP170 has some isoforms with MW 170-200 kDa, 160 kDa and some fragments 30-40 kDa, ~75 kDa.

Publications

What published studies show

The anti-BUB1B antibody (Cat# 18899-1-AP) has 5 total citations. Citations appear in Signal Transduction and Targeted Therapy, Molecular Cancer, Acta Pharmaceutica Sinica B, Cell Insight, and Theriogenology. Research fields include multiple myeloma malignancy, chromosomal instability, bone lesion formation, cell proliferation via mRNA acetylation, genomic instability prevention, and mouse spermatogenesis/male fertility. Applications span WB, IP, IF, and IHC in human and mouse samples.

Automatically generated from the 5 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IP	Signal Transduct Target Ther; BUB1B and circBUB1B_544aa aggravate multiple myeloma malignancy through evoking chromosomal instability.; Authors - Xiaozhu Tang ; KD Validated
human	WB,IP,IF	Mol Cancer; CHEK1 and circCHEK1_246aa evoke chromosomal instability and induce bone lesion formation in multiple myeloma.; Authors - Chunyan Gu
human	WB	Acta Pharm Sin B; NAT10 promotes cell proliferation by acetylating CEP170 mRNA to enhance translation efficiency in multiple myeloma; Authors - Rongfang Wei
human	WB,IP	Cell Insight; LUC7L3 is a downstream factor of SRSF1 and prevents genomic instability; Authors - Xiaqing Zhang
mouse	WB,IHC	Theriogenology; Centromere protein CEP170 is dispensable for mouse spermatogenesis and male fertility.; Authors - Jie Li ; KO Validated