CEP192 Polyclonal antibody

CEP192 Polyclonal Antibody for IF, IHC, IP,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 18832-1-AP

Synonyms

centrosomal protein 192kDa, CEP192, KIAA1569, KIAA1569 protein



Tested Applications

Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 5 publications below

Product Information

18832-1-AP targets CEP192 in IP, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name centrosomal protein 192kDa
Calculated molecular weight 213 kDa
Observed molecular weight 250-300 kDa
GenBank accession numberBC144481
Gene symbol CEP192
Gene ID (NCBI) 55125
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CEP192, also named as PP8407 and KIAA1569, interacts with SHBG. It is required for mitotic centrosome and spindle assembly. CEP192 appears to be a major regulator of pericentriolar material (PCM) recruitment, centrosome maturation, and centriole duplication.

Protocols

Product Specific Protocols
IHC protocol for CEP192 antibody 18832-1-APDownload protocol
IF protocol for CEP192 antibody 18832-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Sci Rep

Plk1 Mediates Paxillin Phosphorylation (Ser-272), Centrosome Maturation, and Airway Smooth Muscle Layer Thickening in Allergic Asthma.

Authors - Alyssa C Rezey
humanIF

Cell Rep

CCDC57 Cooperates with Microtubules and Microcephaly Protein CEP63 and Regulates Centriole Duplication and Mitotic Progression.

Authors - H Kubra Gurkaslar
mouseIF

PLoS Genet

Aurora kinase A is essential for meiosis in mouse oocytes.

Authors - Cecilia S Blengini
humanIF

RNA

Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.

Authors - Alan L Jiao
mouseIF

Science

A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.

Authors - Chun So