CEP192 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• CEP192 Polyclonal antibody (28700-1-AP)

CEP192 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 28700-1-AP Copied!

Publications (2)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

centrosomal protein 192kDa, Centrosomal protein of 192 kDa, Cep192/SPD-2, KIAA1569, PP8407

• 

  WB analysis using 28700-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28700-1-AP (CEP192 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary cancer using 28700-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28700-1-AP (CEP192 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 28700-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28700-1-AP (CEP192 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 28700-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 28700-1-AP (CEP192 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 28700-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP192 antibody (28700-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for CEP192 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28700-1-AP targets CEP192 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29930

  Product name: Recombinant human CEP192 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 768-988 aa of NM_032142

  Sequence: NDLEKSNGSNALDMEKYLKKTEVSRYESALENFSRASMSDTWDLSLPKEQTTQDIHPVDLSATSVSVRAPEENTAAIVYVENGESENQESFRTINSSNSVTNRENNSAVVDVKTCSIDNKLQDVGNDEKATSISTPSDSYSSVRNPRITSLCLLKDCEEIRDNRENQRQNECVSEISNSEKHVTFENHRIVSPKNSDLKNTSPEHGGRGSEDEQESFRPST

• Full Name: centrosomal protein 192kDa
• Calculated Molecular Weight: 297 kDa
• Observed Molecular Weight: 280-300 kDa
• GenBank Accession Number: NM_032142
• Gene Symbol: CEP192
• Gene ID (NCBI): 55125
• RRID: AB_2918191
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TEP8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP192, also named PP8407 and KIAA1569, interacts with SHBG. It is required for mitotic centrosome and spindle assembly. CEP192 appears to be a major regulator of pericentriolar material (PCM) recruitment, centrosome maturation, and centriole duplication.

Publications

What published studies show

The CEP192 Polyclonal antibody (Cat# 28700-1-AP) has 2 citations. These appear in Nature Communications and EMBO Reports. The cited research focuses on centrosome biology—specifically cell cycle-dependent centrosome maturation and centriole number control through PLK4-dependent phosphorylation pathways. Both studies used the antibody for immunofluorescence (IF) in human samples.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Nat Commun; A cell cycle-dependent transition of acetylation to phosphorylation regulates timely centrosome maturation.; Authors - Jimin Li
human	IF	EMBO Rep; McIdas localizes to centrioles and controls centriole numbers through PLK4-dependent phosphorylation.; Authors - Marina Arbi