Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, mouse testis tissue |
| Positive IHC detected in | mouse adrenal gland tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67460-1-Ig targets CEP55 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29995 Product name: Recombinant human CEP55 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-355 aa of BC008947 Sequence: MSSRSTKDLIKSKWGSKPSNSKSETTLEKLKGEIAHLKTSVDEITSGKGKLTDKERHRLLEKIRVLEAEKEKNAYQLTEKDKEIQRLRDQLKARYSTTALLEQLEETTREGERREQVLKALSEEKDVLKQQLSAATSRIAELESKTNTLRLSQTVAPNCFNSSINNIHEMEIQLKDALEKNQQWLVYDQQREVYVKGLLAKIFELEKKTETAAHSLPQQTKKPESEGYLQEEKQKCYNDLLASAKKDLEVERQTITQLSFELSEFRRKYEETQKEVHNLNQLLYSQRRADVQHLEDDRHKTEKIQKLREENDIARGKLEEEKKRSEELLSQVQFLYTSLLKQQEEQTRVALLEQQ Predict reactive species |
| Full Name | centrosomal protein 55kDa |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 47-55 kDa |
| GenBank Accession Number | BC008947 |
| Gene Symbol | CEP55 |
| Gene ID (NCBI) | 55165 |
| RRID | AB_2882690 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q53EZ4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CEP55, also named as C10orf3 and URCC6, is a 55 kDa protein which located in the centrosome in interphase cells and recruited to the midbody during cytokinesis, is essential for completion of cell abscission. It plays a role in mitotic exit and cytokinesis.CEP55 has two isoforms with the MW of 47 and 55 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CEP55 antibody 67460-1-Ig | Download protocol |
| IHC protocol for CEP55 antibody 67460-1-Ig | Download protocol |
| WB protocol for CEP55 antibody 67460-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CEP55 Monoclonal antibody (2D11F7), catalog number 67460-1-Ig, has 1 total citation. It was cited in Nature Structural & Molecular Biology in a study investigating how ESCRT-III assembles around mis-segregated DNA to protect genome stability. The research field encompasses genome stability, DNA segregation, and cytokinesis, consistent with CEP55's known role in mitotic exit and cell abscission. The cited application was Western blotting.
| Species | Application | Title |
|---|---|---|
Nat Struct Mol Biol ESCRT-III assembles around mis-segregated DNA to protect genome stability
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