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CEP55 Monoclonal antibody
CEP55 Monoclonal Antibody for IF, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
WB, IF, ELISA
Conjugate
Unconjugated
CloneNo.
2D11F7
Cat no : 67460-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, mouse testis tissue |
Positive IF detected in | HeLa cells, HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
67460-1-Ig targets CEP55 in WB, IF, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | CEP55 fusion protein Ag29995 |
Full Name | centrosomal protein 55kDa |
Calculated molecular weight | 54 kDa |
Observed molecular weight | 47-55 kDa |
GenBank accession number | BC008947 |
Gene symbol | CEP55 |
Gene ID (NCBI) | 55165 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CEP55, also named as C10orf3 and URCC6, is a 55 kDa protein which located in the centrosome in interphase cells and recruited to the midbody during cytokinesis, is essential for completion of cell abscission. It plays a role in mitotic exit and cytokinesis.CEP55 has two isoforms with the MW of 47 and 55 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for CEP55 antibody 67460-1-Ig | Download protocol |
IF protocol for CEP55 antibody 67460-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |