CEP55 Monoclonal antibody, PBS Only

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  WB analysis using 67460-1-Ig (same clone as 67460-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67460-1-Ig (CEP55 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67460-1-Ig (same clone as 67460-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67460-1-Ig (CEP55 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67460-1-Ig (same clone as 67460-1-PBS)

  WB result of CEP55 antibody (67460-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CEP55 transfected HeLa cells. This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse adrenal gland using 67460-1-Ig (same clone as 67460-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 67460-1-Ig (CEP55 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse adrenal gland using 67460-1-Ig (same clone as 67460-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 67460-1-Ig (CEP55 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67460-1-Ig (same clone as 67460-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67460-1-Ig (CEP55 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67460-1-Ig (same clone as 67460-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CEP55 antibody (67460-1-Ig, Clone: 2D11F7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67460-1-Ig (same clone as 67460-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67460-1-Ig (CEP55 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CEP55 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67460-1-PBS

Clone No.2D11F7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Up-regulated in colon cancer 6, Up regulated in colon cancer 6, Centrosomal protein of 55 kDa, centrosomal protein 55kDa, C10orf3

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Product Information

67460-1-PBS targets CEP55 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29995

  Product name: Recombinant human CEP55 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-355 aa of BC008947

  Sequence: MSSRSTKDLIKSKWGSKPSNSKSETTLEKLKGEIAHLKTSVDEITSGKGKLTDKERHRLLEKIRVLEAEKEKNAYQLTEKDKEIQRLRDQLKARYSTTALLEQLEETTREGERREQVLKALSEEKDVLKQQLSAATSRIAELESKTNTLRLSQTVAPNCFNSSINNIHEMEIQLKDALEKNQQWLVYDQQREVYVKGLLAKIFELEKKTETAAHSLPQQTKKPESEGYLQEEKQKCYNDLLASAKKDLEVERQTITQLSFELSEFRRKYEETQKEVHNLNQLLYSQRRADVQHLEDDRHKTEKIQKLREENDIARGKLEEEKKRSEELLSQVQFLYTSLLKQQEEQTRVALLEQQ

• Full Name: centrosomal protein 55kDa
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 47-55 kDa
• GenBank Accession Number: BC008947
• Gene Symbol: CEP55
• Gene ID (NCBI): 55165
• RRID: AB_2882690
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q53EZ4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CEP55, also named as C10orf3 and URCC6, is a 55 kDa protein which located in the centrosome in interphase cells and recruited to the midbody during cytokinesis, is essential for completion of cell abscission. It plays a role in mitotic exit and cytokinesis.CEP55 has two isoforms with the MW of 47 and 55 kDa.