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CEP63 Polyclonal antibody
CEP63 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, canine
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 16268-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, mouse liver tissue, HeLa cells, mouse thymus tissue |
Positive IP detected in | HEK-293 cells |
Positive IF/ICC detected in | MDCK cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 5 publications below |
IF | See 12 publications below |
IP | See 3 publications below |
Product Information
16268-1-AP targets CEP63 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
Tested Reactivity | human, mouse, rat, canine |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CEP63 fusion protein Ag9335 |
Full Name | centrosomal protein 63kDa |
Calculated Molecular Weight | 703 aa, 81 kDa |
Observed Molecular Weight | 57-75 kDa |
GenBank Accession Number | BC014050 |
Gene Symbol | CEP63 |
Gene ID (NCBI) | 80254 |
RRID | AB_2077079 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CEP63 is a 63 kDa centrosomal protein. It is required for normal spindle assembly. It maintains centrosome numbers through centrosomal recruitment of CEP152. CEP63 recruits CDK1 to centrosomes. It plays a role in DNA damage response. This antibody can recognize all the four isoforms of CEP63 (56 kDa, 58 kDa, 63 kDa and 81 kDa).
Protocols
Product Specific Protocols | |
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WB protocol for CEP63 antibody 16268-1-AP | Download protocol |
IF protocol for CEP63 antibody 16268-1-AP | Download protocol |
IP protocol for CEP63 antibody 16268-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Cell Biol Massive centriole production can occur in the absence of deuterosomes in multiciliated cells. | ||
Neuron Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate. | ||
Dev Cell A dual role for UVRAG in maintaining chromosomal stability independent of autophagy.
| ||
Nat Commun Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis. | ||
Nat Commun Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Abe (Verified Customer) (04-25-2024) | Worked well for IF at 1:1000 overnight at 4c in RPE-1 cells.
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