CEP63 Monoclonal antibody

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  WB analysis using 66996-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66996-1-Ig (CEP63 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of hTERT-RPE1 using 66996-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CEP63 antibody (66996-1-Ig, Clone: 2G12B2 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

CEP63 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 66996-1-Ig

Clone No.2G12B2

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

2G12B2, centrosomal protein 63kDa, Centrosomal protein of 63 kDa

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, T-47D cells, PC-3 cells, HepG2 cells, HSC-T6 cells
• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

66996-1-Ig targets CEP63 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9227

  Product name: Recombinant human CEP63 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-349 aa of BC014050

  Sequence: MEALLEGIQNRGHGGGFLTSCEAELQELMKQIDIMVAHKKSEWEGRTHALETCLKIREQELKSLRSQLDVTHKEVGMLHQQVEEHEKIKQEMTMEYKQELKKLHEELCILKRSYEKLQKKQMREFRGNTKNHREDRSEIERLTAKIEEFRQKSLDWEKQRLIYQQQVSSLEAQRKALAEQSEIIQAQLVNRKQKLESVELSSQSEIQHLSSKLERANDTICANELEIERLTMRVNDLVGTSMTVLQEQQQKEEKLRESEKLLEALQEEKRELKAALQSQENLIHEARIQKEKLQEKVKATNTQHAVEAISLESVSATCKQLSQELMEKYEELKRMEAHNNEYKAEIKKL

• Full Name: centrosomal protein 63kDa
• Calculated Molecular Weight: 703 aa, 81 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC014050
• Gene Symbol: CEP63
• Gene ID (NCBI): 80254
• RRID: AB_2882313
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q96MT8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP63 is required for normal spindle assembly. It maintains centrosome numbers through centrosomal recruitment of CEP152. CEP63 recruits CDK1 to centrosomes. It plays a role in DNA damage response.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Neural Regen Res; CNKSR2 interactome analysis indicates its association with the centrosome/microtubule system; Authors - Lin Yin