CEP68 Polyclonal antibody

• Featured Product
• KD/KO Validated

CEP68 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 15147-1-AP

Synonyms

centrosomal protein 68kDa, Centrosomal protein of 68 kDa, CEP68, KIAA0582

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Validation Data Gallery

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  WB analysis using 15147-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15147-1-AP (CEP68 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15147-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15147-1-AP (CEP68 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15147-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15147-1-AP (CEP68 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HeLa cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IF: See 6 publications below

Product Information

15147-1-AP targets CEP68 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEP68 fusion protein Ag7447
• Full Name: centrosomal protein 68kDa
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC002982
• Gene Symbol: CEP68
• Gene ID (NCBI): 23177
• RRID: AB_2077081
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	J Cell Sci; Cep44 functions in centrosome cohesion by stabilizing rootletin; Authors - Delowar Hossain ; KD Validated
human	WB	J Virol; The Cytoskeletal Adaptor Obscurin-Like 1 Interacts with the Human Papillomavirus 16 (HPV16) Capsid Protein L2 and Is Required for HPV16 Endocytosis.; Authors - Elena Wüstenhagen ; KD Validated
human	IF	Sci Rep; Prolonged overexpression of PLK4 leads to formation of centriole rosette clusters that are connected via canonical centrosome linker proteins; Authors - Selahattin Can Ozcan
human	WB,IF	J Cell Sci; Cep44 functions in centrosome cohesion by stabilizing rootletin.; Authors - Delowar Hossain ; KD Validated
human	WB	RNA; Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.; Authors - Alan L Jiao
human	WB,IF	iScience; Pathogenic LRRK2 regulates centrosome cohesion via Rab10/RILPL1-mediated CDK5RAP2 displacement.; Authors - Elena Fdez