CEP68 Polyclonal antibody

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  WB analysis using 15147-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15147-1-AP (CEP68 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15147-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15147-1-AP (CEP68 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15147-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15147-1-AP (CEP68 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

CEP68 Polyclonal Antibody for WB, ELISA

Cat No. 15147-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Synonyms

Centrosomal protein of 68 kDa

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HeLa cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IF: See 6 publications below

Product Information

15147-1-AP targets CEP68 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEP68 fusion protein Ag7447
• Full Name: centrosomal protein 68kDa
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 90 kDa, 67 kDa
• GenBank Accession Number: BC002982
• Gene Symbol: CEP68
• Gene ID (NCBI): 23177
• RRID: AB_2077081
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q76N32
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP68, or centrosomal protein 68, is a protein-coding gene in humans. It is involved in the maintenance of centrosome cohesion, likely as part of a linker structure that prevents centrosome splitting. CEP68 is also required for the localization of CDK5RAP2 to the centrosome during interphase and contributes to the formation of CROCC/rootletin filaments. This protein is encoded by the CEP68 gene and is located on the fibers emanating from the proximal ends of centrioles. It has been associated with diseases such as Retinitis Pigmentosa 28 and is implicated in angioedema. The gene is mapped to chromosome 2 and is expressed ubiquitously in various tissues, including the brain, ovary, and others. The protein plays a crucial role in cellular functions related to the centrosome, which is a key organelle in cell division and organization. This antibody recognized two bands of ∼90 and 67 kDa, respectively, in good agreement with the predicted sizes of the two Cep68 isoforms (81 and 67 kDa for Cep68L and -S, respectively).

Publications

Species	Application	Title
human	WB,IF	J Cell Sci; Cep44 functions in centrosome cohesion by stabilizing rootletin; Authors - Delowar Hossain ; KD Validated
human	WB	J Virol; The Cytoskeletal Adaptor Obscurin-Like 1 Interacts with the Human Papillomavirus 16 (HPV16) Capsid Protein L2 and Is Required for HPV16 Endocytosis.; Authors - Elena Wüstenhagen ; KD Validated
human	IF	Sci Rep; Prolonged overexpression of PLK4 leads to formation of centriole rosette clusters that are connected via canonical centrosome linker proteins; Authors - Selahattin Can Ozcan
human	WB,IF	J Cell Sci; Cep44 functions in centrosome cohesion by stabilizing rootletin.; Authors - Delowar Hossain ; KD Validated
human	WB	RNA; Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.; Authors - Alan L Jiao
human	WB,IF	iScience; Pathogenic LRRK2 regulates centrosome cohesion via Rab10/RILPL1-mediated CDK5RAP2 displacement.; Authors - Elena Fdez