CER1 Polyclonal antibody

CER1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 18879-1-AP

Synonyms

CER1, Cerberus, Cerberus related protein, DAN domain family member 4, DAND4

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Validation Data Gallery

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  WB analysis using 18879-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18879-1-AP (CER1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: U-251 cells, mouse kidney tissue, rat kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18879-1-AP targets CER1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CER1 fusion protein Ag12926
• Full Name: cerberus 1, cysteine knot superfamily, homolog (Xenopus laevis)
• Calculated Molecular Weight: 267 aa, 30 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC069405
• Gene Symbol: CER1
• Gene ID (NCBI): 9350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CER1 is a cytokine member of the cysteine knot superfamily, characterized by nine conserved cysteines and a cysteine knot region. The cerberus-related cytokines, together with Dan and DRM/Gremlin, represent a group of bone morphogenetic protein (BMP) antagonists that can bind directly to BMPs and inhibit their activity. CER1 can exist as homo- or heterodimers (PMID: 23717584, 9501024).