CES1 Polyclonal antibody

CES1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29041-1-AP

Synonyms

ACAT, Brain carboxylesterase hBr1, CEH, CES1, CES2, Cocaine carboxylesterase, Egasyn, HMSE, HMSE1, Liver carboxylesterase 1, PCE 1, REH, Retinyl ester hydrolase, Serine esterase 1, SES1, TGH, Triacylglycerol hydrolase

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Validation Data Gallery

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  WB analysis using 29041-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29041-1-AP (CES1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29041-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29041-1-AP (CES1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 29041-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29041-1-AP (CES1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of C2C12 using 29041-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CES1 antibody (29041-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 29041-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CES1 antibody (29041-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of C2C12 using 29041-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CES1 antibody (29041-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: THP-1 cells, rat liver tissue, hepG2 cells, Caco-2 cells
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: C2C12 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29041-1-AP targets CES1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CES1 fusion protein Ag30534
• Full Name: carboxylesterase 1 (monocyte/macrophage serine esterase 1)
• Calculated Molecular Weight: 566 aa, 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC012418
• Gene Symbol: CES1
• Gene ID (NCBI): 1066
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human carboxylesterase 1 (hCE1 or CES1) is a serine esterase that hydrolyzes a variety of endogenous and exogenous substrates. CES1 contributes to approximately 90% of hepatic hydrolytic activity in human livers (PMID: 27895113). Variation in CES1 has the ability to affect the metabolism of methylphenidate (PMID: 28087982). CES1 has 567 amino acids and a theoretical molecular mass of 62 kDa.