CES7-Specific Polyclonal antibody

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  WB analysis of HepG2 using 19520-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19520-1-AP (CES7-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 19520-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 19520-1-AP (CES7-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

CES7-Specific Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 19520-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Carboxylesterase 7, CES7, Carboxylesterase 5A, CES5, CES4C1

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19520-1-AP targets CES7-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: carboxylesterase 7
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 58-60 kDa
• GenBank Accession Number: NM_145024
• Gene Symbol: CES7
• Gene ID (NCBI): 221223
• RRID: AB_10642002
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6NT32
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CES7, also named as CAUXIN, CES4C1, CES5 and FLJ31547, belongs to the type-B carboxylesterase/lipase family. CES7 is involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. CES7 catalyze the reaction: A carboxylic ester + H2O = an alcohol + a carboxylate. The antibody can recognize the isoform1 and isoform2.