Complement factor D Polyclonal antibody

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  WB analysis of human plasma using 26050-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 26050-1-AP (Complement factor D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 26050-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 26050-1-AP (Complement factor D Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of human stomach using 26050-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 26050-1-AP (Complement factor D antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

Complement factor D Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26050-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CFD, EC:3.4.21.46, DF, complement factor D (adipsin), C3 convertase activator

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Tested Applications

• Positive WB detected in: human plasma tissue, human placenta tissue, human plasma
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

26050-1-AP targets Complement factor D in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Complement factor D fusion protein Ag23473
• Full Name: complement factor D (adipsin)
• Calculated Molecular Weight: 253 aa, 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC057807
• Gene Symbol: CFD
• Gene ID (NCBI): 1675
• RRID: AB_2880352
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P00746
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Complement factor D, also known as adipsin, is a highly specific serine protease. Complement factor D is the rate-limiting enzyme in the alternative pathway of complement. It activates C3 convertase by cleaving factor B, which is a key step in the alternative pathway. Complement factor D catalyzes the cleavage of factor B to generate Ba (non-catalytic chain) and Bb (active catalytic subunit), which together with C3(H2O) (or C3b) constitute the active C3 convertase C3(H2O)Bb (or C3bBb). (PMID: 34566967; PMID: 27775713).

Publications

Species	Application	Title
human	WB	Immunol Res; The influences of α-hemolytic Streptococcus on class switching and complement activation of human tonsillar cells in IgA nephropathy.; Authors - Muyao Ye
human	WB	Hypertens Res; Endothelial-derived complement factor D contributes to endothelial dysfunction in malignant nephrosclerosis via local complement activation; Authors - Zheng Wang ; KD Validated