CFHR2 Polyclonal antibody

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  WB analysis using 24048-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 24048-1-AP (CFHR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 24048-1-AP

  IP result of anti-CFHR2 (IP:24048-1-AP, 4ug; Detection:24048-1-AP 1:1000) with HepG2 cells lysate 1800 ug.

CFHR2 Polyclonal Antibody for WB, IP, ELISA

Cat No. 24048-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

CFHL2, complement factor H related 2, DDESK59, FHR 2, FHR2

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Tested Applications

• Positive WB detected in: HuH-7 cells
• Positive IP detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24048-1-AP targets CFHR2 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21106

  Product name: Recombinant human CFHR2 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-270 aa of BC022283

  Sequence: MWLLVSVILISRISSVGGEAMFCDFPKINHGILYDEEKYKPFSQVPTGEVFYYSCEYNFVSPSKSFWTRITCAEEGWSPTPKCLRLCFFPFVENGHSESSGQTHLEGDTVQIICNTGYRLQNNENNISCVERGWSTPPKCRSTISAEKCGPPPPIDNGDITSFLLSVYAPGSSVEYQCQNLYQLEGNNQITCRNGQWSEPPKCLDPCVISQEIMEKYNIKLKWTNQQKLYSRTGDIVEFVCKSGYHPTKSHSFRAMCQNGKLVYPSCEEK

• Full Name: complement factor H-related 2
• Calculated Molecular Weight: 270 aa, 31 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC022283
• Gene Symbol: CFHR2
• Gene ID (NCBI): 3080
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: P36980
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Complement factor H-related protein 2 (CFHR2) is a human immune-related recombinant protein and a member of the family that assists in the activation of the complement system. This protein originates from the filtration membrane of the glomerulus and the lymphatic system, and plays an important role in immune regulation and inflammation suppression.