Cofilin 2 Polyclonal antibody

Cofilin 2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11848-1-AP

Synonyms

CFL2, CFL 2, NEM7, Cofilin-2, Cofilin2

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Validation Data Gallery

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  WB analysis of human liver using 11848-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11848-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11848-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 11848-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11848-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 11848-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 11848-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 11848-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 11848-1-AP (Cofilin 2 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human normal colon using 11848-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 11848-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11848-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 11848-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11848-1-AP (Cofilin 2 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 11848-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11848-1-AP (Cofilin 2 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 11848-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 11848-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of L02 using 11848-1-AP

  Immunofluorescent analysis of L02 cells using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human liver tissue, L02 cells, mouse liver tissue, mouse lung tissue, mouse skeletal muscle tissue
• Positive IHC detected in: human normal colon, human gliomas tissue, human heart tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

11848-1-AP targets Cofilin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cofilin 2 fusion protein Ag2408
• Full Name: cofilin 2 (muscle)
• Calculated Molecular Weight: 166 aa, 19 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC022876
• Gene Symbol: Cofilin 2
• Gene ID (NCBI): 1073
• RRID: AB_2080914
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Mol Ther; Intercellular transfer of miR-200c-3p impairs the angiogenic capacity of cardiac endothelial cells.; Authors - Lara Ottaviani
human	WB,IHC,IF	Noncoding RNA Res; ADAR1-regulated miR-142-3p/RIG-I axis suppresses antitumor immunity in nasopharyngeal carcinoma; Authors - Haoyuan Xu