Cofilin 2 Polyclonal antibody

Cofilin 2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11848-1-AP

Synonyms

CFL2, Cofilin 2, cofilin 2 (muscle), Cofilin, muscle isoform, NEM7



Tested Applications

Positive WB detected inhuman liver tissue, L02 cells, mouse liver tissue, mouse lung tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue, human heart tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11848-1-AP targets Cofilin 2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cofilin 2 fusion protein Ag2408
Full Name cofilin 2 (muscle)
Calculated Molecular Weight 166 aa, 19 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC022876
Gene Symbol CFL2
Gene ID (NCBI) 1073
RRIDAB_2080914
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Cofilin 2 antibody 11848-1-APDownload protocol
IHC protocol for Cofilin 2 antibody 11848-1-APDownload protocol
IF protocol for Cofilin 2 antibody 11848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Ther

Intercellular transfer of miR-200c-3p impairs the angiogenic capacity of cardiac endothelial cells.

Authors - Lara Ottaviani