CGRRF1 Polyclonal antibody

• 

  WB analysis of rat kidney using 16101-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 16101-1-AP (CGRRF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 16101-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16101-1-AP (CGRRF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 16101-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 16101-1-AP (CGRRF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

CGRRF1 Polyclonal Antibody for WB, ELISA

Cat No. 16101-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

CGR19, CGRRF1, RING finger protein 197, RNF197

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Tested Applications

• Positive WB detected in: rat kidney tissue, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16101-1-AP targets CGRRF1 in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8998

  Product name: Recombinant human CGRRF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 42-332 aa of BC015063

  Sequence: VILRNSEETQFSTRVFKKQMRQVKNPFGLEITNPSSASITTGITLTTDCLEDSLLTCYWGCNVQKLYEALQKHVYCFRISTPQALEDALYSEYLYQEQYFIKKDSKEEIYCQLPRDTKIEDFGTVPRSRYPLVALLTLADEDDREIYDIISMVSVIHIPDRTYKLSCRILYQYLLLAQGQFHDLKQLFMSANNNFTPSNNSSSEEKNTDRSLLEKVGLSESEVEPSEENSKDCVVCQNGTVNWVLLPCRHTCLCDGCVKYFQQCPMCRQFVQESFALCSQKEQDKDKPKTL

• Full Name: cell growth regulator with ring finger domain 1
• Calculated Molecular Weight: 332 aa, 38 kDa
• Observed Molecular Weight: 42-48 kDa
• GenBank Accession Number: BC015063
• Gene Symbol: CGRRF1
• Gene ID (NCBI): 10668
• RRID: AB_2878219
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99675
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CGRRF1 is a growth suppressor and consists of a transmembrane domain and a RING-finger domain. It functions as a RING domain E3 ubiquitin ligase involved in endoplasmic reticulum-associated degradation. The expression of CGRRF1 is decreased in cancer tissues.(PMID: 31801577).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Neoplasia; A novel regulatory axis of MSI2-AGO2/miR-30a-3p-CGRRF1 drives cancer chemoresistance by upregulating the KRAS/ERK pathway; Authors - Runhui Lu