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  • KD/KO Validated

CHAF1B Polyclonal antibody

CHAF1B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 27633-1-AP

Synonyms

CAF 1, CAF 1 subunit B, CAF I 60 kDa subunit, CAF I p60, CAF IP60, CAF1, CAF1A, CAF1P60, CHAF1B, M phase phosphoprotein 7, MPHOSPH7, MPP7



Tested Applications

Positive WB detected inCaco-2 cells, HeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27633-1-AP targets CHAF1B in WB, ChIP, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHAF1B fusion protein Ag26504
Full Name chromatin assembly factor 1, subunit B (p60)
Calculated Molecular Weight 559 aa, 61 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC021218
Gene Symbol CHAF1B
Gene ID (NCBI) 8208
RRIDAB_2880933
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHAF1B, also named as CAF1A, CAF1P60, MPHOSPH7, MPP7, is a 559 amino acid protein, which belongs to the WD repeat HIR1 family. CHAF1B as a subunit of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1B is thought to mediate chromatin assembly in DNA replication and DNA repair and assembles histone octamers onto replicating DNA in vitro. The calcualted molecular weight of CHAF1B is 61 kDa, but molecular weight of CHAF1B is detected about 65-70 kDa.

Protocols

Product Specific Protocols
WB protocol for CHAF1B antibody 27633-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomed Pharmacother

Affinity-based protein profiling to reveal targets of puerarin involved in its protective effect on cardiomyocytes.

Authors - Shuai Huang
  • KD Validated
humanChIP

Cancer Res Commun

Targeting CHAF1B Enhances IFN Activity against Myeloproliferative Neoplasm Cells

Authors - Diana Saleiro