CHAF1B Polyclonal antibody

• Featured Product
• KD/KO Validated

CHAF1B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ChIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 27633-1-AP

Synonyms

MPP 7, p60 CAF1, CAF1, CAF IP60, CAF I p60

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Validation Data Gallery

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  WB analysis using 27633-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 27633-1-AP (CHAF1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27633-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27633-1-AP (CHAF1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 27633-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27633-1-AP (CHAF1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 27633-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27633-1-AP (CHAF1B antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 27633-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27633-1-AP (CHAF1B antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Caco-2 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• ChIP: See 1 publications below

Product Information

27633-1-AP targets CHAF1B in WB, IHC, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHAF1B fusion protein Ag26504
• Full Name: chromatin assembly factor 1, subunit B (p60)
• Calculated Molecular Weight: 559 aa, 61 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC021218
• Gene Symbol: CHAF1B
• Gene ID (NCBI): 8208
• RRID: AB_2880933
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHAF1B, also named as CAF1A, CAF1P60, MPHOSPH7, MPP7, is a 559 amino acid protein, which belongs to the WD repeat HIR1 family. CHAF1B as a subunit of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1B is thought to mediate chromatin assembly in DNA replication and DNA repair and assembles histone octamers onto replicating DNA in vitro. The calcualted molecular weight of CHAF1B is 61 kDa, but molecular weight of CHAF1B is detected about 65-70 kDa.

Publications

Species	Application	Title
human	WB	Biomed Pharmacother; Affinity-based protein profiling to reveal targets of puerarin involved in its protective effect on cardiomyocytes.; Authors - Shuai Huang ; KD Validated
human	ChIP	Cancer Res Commun; Targeting CHAF1B Enhances IFN Activity against Myeloproliferative Neoplasm Cells; Authors - Diana Saleiro