CHAT Polyclonal antibody, PBS Only

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  WB analysis using 20747-1-AP (same clone as 20747-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20747-1-AP (CHAT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse placenta using 20747-1-AP (same clone as 20747-1-PBS)

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 20747-1-AP (CHAT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

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  IP experiment of rat brain using 20747-1-AP (same clone as 20747-1-PBS)

  IP result of anti-CHAT (IP:20747-1-AP, 4ug; Detection:20747-1-AP 1:500) with rat brain tissue lysate 4000ug. This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 20747-1-AP (same clone as 20747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20747-1-AP (CHAT antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 20747-1-AP (same clone as 20747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20747-1-AP (CHAT antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 20747-1-AP (same clone as 20747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20747-1-AP (CHAT antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 20747-1-AP (same clone as 20747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20747-1-AP (CHAT antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 20747-1-AP (same clone as 20747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20747-1-AP (CHAT antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 20747-1-AP (same clone as 20747-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CHAT antibody (20747-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

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  IF Staining of Neuro-2a using 20747-1-AP (same clone as 20747-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using CHAT antibody (20747-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

CHAT Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 20747-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

CHOACTase, Choline acetylase, choline acetyltransferase, Choline O-acetyltransferase, EC:2.3.1.6

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Product Information

20747-1-PBS targets CHAT in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: choline acetyltransferase
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 47-50 kDa, 66-70 kDa
• GenBank Accession Number: NM_020549
• Gene Symbol: CHAT
• Gene ID (NCBI): 1103
• RRID: AB_10898169
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P28329
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CHAT (choline acetyltransferase) belongs to the carnitine/choline acetyltransferase family. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA). CHAT is known to be present exclusively in the cytoplasm of cholinergic neurons and anti-CHAT antibody has been considered as the best marker for cholinergic neurons. Human CHAT gene generates multiple mRNA splice variants (PMID: 10861222). All these variants possess start codons for translation of 70 kDa cChAT protein (isoform 1). The M variant has an additional start codon for 83 kDa cChAT protein (isoform 2), which has been found in nuclei of neuronal cells in human CNS; and the S variant has another codon for 74 kDa cChAT protein (isoform 3). Besides, CHAT includes a short exon-skipping splice variant (peripheral type CHAT) pCHAT encoding 40-55 kDa (PMID: 31040770, 29759072). This antibody can recognize both pCHAT and cCHAT.