CHCHD3 Polyclonal antibody
CHCHD3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 25625-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, human placenta tissue, A431 cells, mouse liver tissue, NIH/3T3 cells, Jurkat cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:4000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 14 publications below |
| IF | See 2 publications below |
Product Information
The immunogen of 25625-1-AP is CHCHD3 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CHCHD3 fusion protein Ag22579 |
| Full Name | coiled-coil-helix-coiled-coil-helix domain containing 3 |
| Calculated molecular weight | 227 aa, 26 kDa |
| Observed molecular weight | 26 kDa |
| GenBank accession number | BC014839 |
| Gene symbol | CHCHD3 |
| Gene ID (NCBI) | 54927 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CHCHD3 antibody 25625-1-AP | Download protocol |
| IHC protocol for CHCHD3 antibody 25625-1-AP | Download protocol |
| IF protocol for CHCHD3 antibody 25625-1-AP | Download protocol |
| IP protocol for CHCHD3 antibody 25625-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun ATAD3A oligomerization causes neurodegeneration by coupling mitochondrial fragmentation and bioenergetics defects. | ||
Acta Neuropathol Alpha-synuclein suppresses mitochondrial protease ClpP to trigger mitochondrial oxidative damage and neurotoxicity. | ||
Biochim Biophys Acta Cardiolipin synthesizing enzymes form a complex that interacts with cardiolipin-dependent membrane organizing proteins. | ||
Life Sci Alliance MIC26 and MIC27 cooperate to regulate cardiolipin levels and the landscape of OXPHOS complexes. | ||
Biochim Biophys Acta Biomembr Conserved GxxxG and WN motifs of MIC13 are essential for bridging two MICOS subcomplexes. | ||
J Proteome Res Mapping Interactome Networks of DNAJC11, a Novel Mitochondrial Protein Causing Neuromuscular Pathology in Mice. |
