Validation Data Gallery
|Positive WB detected in||HEK-293 cells, human placenta tissue, A431 cells, mouse liver tissue, HeLa cells, Jurkat cells, NIH/3T3 cells, NIH/3T3 cells.|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human liver tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:4000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 25625-1-AP is CHCHD3 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CHCHD3 fusion protein Ag22579|
|Full Name||coiled-coil-helix-coiled-coil-helix domain containing 3|
|Calculated molecular weight||227 aa, 26 kDa|
|Observed molecular weight||26 kDa|
|GenBank accession number||BC014839|
|Gene ID (NCBI)||54927|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CHCHD3, initially identified as a substrate for cAMP-dependent protein kinase (PKA), is a ubiquitous protein in the mitochondria and plays a prominent role in maintaining cristae integrity and mitochondrial function. In mitochondria, ChChd3 is predominantly localized to the inner membrane (IM), facing toward the intermembrane space (IMS), and is part of the large protein complex now called as MINOS (mitochondrial inner membrane organizing system), or MIB (mitochondrial intermembrane space bridging) CHCHD3 is highly conserved in mammals with human and mouse protein sharing ∼92% sequence similarity.
|Product Specific Protocols|
|WB protocol for CHCHD3 antibody 25625-1-AP||Download protocol|
|IHC protocol for CHCHD3 antibody 25625-1-AP||Download protocol|
|IF protocol for CHCHD3 antibody 25625-1-AP||Download protocol|
|IP protocol for CHCHD3 antibody 25625-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Mitochondria, ER, and nuclear membrane defects reveal early mechanisms for upper motor neuron vulnerability with respect to TDP-43 pathology.
Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria.
Mitochondrial membrane proteins and VPS35 orchestrate selective removal of mtDNA
Cristae undergo continuous cycles of membrane remodelling in a MICOS-dependent manner.
Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control.
Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure.
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Mia (Verified Customer) (09-28-2022)