CHCHD3 Polyclonal antibody

CHCHD3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 25625-1-AP

Synonyms

CHCHD3, MIC19, MINOS3

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Validation Data Gallery

• 

  WB analysis using 25625-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25625-1-AP (CHCHD3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 25625-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 25625-1-AP (CHCHD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 25625-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25625-1-AP (CHCHD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 25625-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25625-1-AP (CHCHD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 25625-1-AP

  IP Result of anti-CHCHD3 (IP:25625-1-AP, 4ug; Detection:25625-1-AP 1:1000) with HeLa cells lysate 2600ug.

• 

  IHC staining of human liver using 25625-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25625-1-AP (CHCHD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 25625-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25625-1-AP (CHCHD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 25625-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25625-1-AP (CHCHD3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 25625-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25625-1-AP (CHCHD3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 25625-1-AP

  Immunofluorescent analysis of HepG2 cells using 25625-1-AP (CHCHD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human placenta tissue, A431 cells, mouse liver tissue, NIH/3T3 cells, Jurkat cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:4000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 16 publications below
• IF: See 2 publications below

Product Information

The immunogen of 25625-1-AP is CHCHD3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHCHD3 fusion protein Ag22579
• Full Name: coiled-coil-helix-coiled-coil-helix domain containing 3
• Calculated molecular weight: 227 aa, 26 kDa
• Observed molecular weight: 26 kDa
• GenBank accession number: BC014839
• Gene symbol: CHCHD3
• Gene ID (NCBI): 54927
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
human	WB	Nat Commun; ATAD3A oligomerization causes neurodegeneration by coupling mitochondrial fragmentation and bioenergetics defects.; Authors - Yuanyuan Zhao
mouse	WB	Acta Neuropathol; Alpha-synuclein suppresses mitochondrial protease ClpP to trigger mitochondrial oxidative damage and neurotoxicity.; Authors - Di Hu
human	WB	Biochim Biophys Acta; Cardiolipin synthesizing enzymes form a complex that interacts with cardiolipin-dependent membrane organizing proteins.; Authors - Mauro Serricchio
	WB	Life Sci Alliance; MIC26 and MIC27 cooperate to regulate cardiolipin levels and the landscape of OXPHOS complexes.; Authors - Ruchika Anand
human	WB	Biochim Biophys Acta Biomembr; Conserved GxxxG and WN motifs of MIC13 are essential for bridging two MICOS subcomplexes.; Authors - Jennifer Urbach
human	WB	J Proteome Res; Mapping Interactome Networks of DNAJC11, a Novel Mitochondrial Protein Causing Neuromuscular Pathology in Mice.; Authors - Foteini Violitzi

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