Tested Applications
| Positive WB detected in | HEK-293 cells, human placenta tissue, HeLa cells, Jurkat cells, NIH/3T3 cells, NIH/3T3 cells., mouse liver tissue, rat liver tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:4000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25625-1-AP targets CHCHD3/MIC19 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22579 Product name: Recombinant human CHCHD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-227 aa of BC014839 Sequence: MGGTTSTRRVTFEADENENITVVKGIRLSENVIDRMKESSPSGSKSQRYSGAYGASVSDEELKRRVAEELALEQAKKESEDQKRLKQAKELDRERAAANEQLTRAILRERICSEEERAKAKHLARQLEEKDRVLKKQDAFYKEQLARLEERSSEFYRVTTEQYQKAAEEVEAKFKRYESHPVCADLQAKILQCYRENTHQTLKCSALATQYMHCVNHAKQSMLEKGG Predict reactive species |
| Full Name | coiled-coil-helix-coiled-coil-helix domain containing 3 |
| Calculated Molecular Weight | 227 aa, 26 kDa |
| Observed Molecular Weight | 26 kDa |
| GenBank Accession Number | BC014839 |
| Gene Symbol | CHCHD3 |
| Gene ID (NCBI) | 54927 |
| RRID | AB_2687533 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NX63 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CHCHD3, initially identified as a substrate for cAMP-dependent protein kinase (PKA), is a ubiquitous protein in the mitochondria and plays a prominent role in maintaining cristae integrity and mitochondrial function. In mitochondria, ChChd3 is predominantly localized to the inner membrane (IM), facing toward the intermembrane space (IMS), and is part of the large protein complex now called as MINOS (mitochondrial inner membrane organizing system), or MIB (mitochondrial intermembrane space bridging) CHCHD3 is highly conserved in mammals with human and mouse protein sharing ∼92% sequence similarity.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CHCHD3/MIC19 antibody 25625-1-AP | Download protocol |
| IHC protocol for CHCHD3/MIC19 antibody 25625-1-AP | Download protocol |
| IP protocol for CHCHD3/MIC19 antibody 25625-1-AP | Download protocol |
| WB protocol for CHCHD3/MIC19 antibody 25625-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CHCHD3/MIC19 Polyclonal antibody (Cat# 25625-1-AP) has 47 citations across journals including Nature Communications, Molecular Cell, Acta Neuropathologica, Cell Reports, FASEB Journal, Journal of Biological Chemistry, Science Advances, Brain, and iScience, among others. Research fields span mitochondrial biology (cristae structure, MICOS complex), neurodegeneration (Alzheimer's, ALS, frontotemporal dementia), metabolism, cancer, cardiology, and inflammation.
| Species | Application | Title |
|---|---|---|
Nat Commun Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis | ||
Nat Commun ATAD3A oligomerization causes neurodegeneration by coupling mitochondrial fragmentation and bioenergetics defects. | ||
Nat Commun Mitochondrial membrane proteins and VPS35 orchestrate selective removal of mtDNA | ||
Nat Commun Mic19 depletion impairs endoplasmic reticulum-mitochondrial contacts and mitochondrial lipid metabolism and triggers liver disease
| ||
Mol Cell Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria. |
Reviews
What customers say
Both reviewers gave 5-star ratings for Western Blot applications on mouse samples. One user reported a clear and specific band in mouse heart tissue and confirmed the antibody also performed well with native PAGE blue gel. The other user, using a 1:1000 dilution on mouse tissue, simply noted it worked well. Overall, the reviews reflect consistent satisfaction with the antibody's specificity and performance in WB experiments on mouse tissues.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Xinyi (Verified Customer) (08-28-2026) | WB results showed a clear and specific band in our mouse heart tissue. Also we have tried native page blue gel, it also works beautifully.
|
Mia (Verified Customer) (09-28-2022) | goood！
|
