CHCHD3/MIC19 Polyclonal antibody

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  WB analysis using 25625-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25625-1-AP (CHCHD3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 25625-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25625-1-AP (CHCHD3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 25625-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 25625-1-AP (CHCHD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 25625-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25625-1-AP (CHCHD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 25625-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25625-1-AP (CHCHD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 25625-1-AP

  IP result of anti-CHCHD3 (IP:25625-1-AP, 4ug; Detection:25625-1-AP 1:1000) with HeLa cells lysate 2600ug.

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  IHC staining of human liver using 25625-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25625-1-AP (CHCHD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 25625-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25625-1-AP (CHCHD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 25625-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25625-1-AP (CHCHD3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 25625-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25625-1-AP (CHCHD3 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 25625-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD3 antibody (25625-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 25625-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD3 antibody (25625-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

CHCHD3/MIC19 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 25625-1-AP

Publications(34)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

CHCHD3, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3, MIC19, MICOS complex subunit MIC19, MINOS3

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Tested Applications

• Positive WB detected in: HEK-293 cells, human placenta tissue, A431 cells, mouse liver tissue, HeLa cells, Jurkat cells, NIH/3T3 cells, NIH/3T3 cells.
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:4000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 32 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

25625-1-AP targets CHCHD3/MIC19 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHCHD3/MIC19 fusion protein Ag22579
• Full Name: coiled-coil-helix-coiled-coil-helix domain containing 3
• Calculated Molecular Weight: 227 aa, 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC014839
• Gene Symbol: CHCHD3
• Gene ID (NCBI): 54927
• RRID: AB_2687533
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NX63
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHCHD3, initially identified as a substrate for cAMP-dependent protein kinase (PKA), is a ubiquitous protein in the mitochondria and plays a prominent role in maintaining cristae integrity and mitochondrial function. In mitochondria, ChChd3 is predominantly localized to the inner membrane (IM), facing toward the intermembrane space (IMS), and is part of the large protein complex now called as MINOS (mitochondrial inner membrane organizing system), or MIB (mitochondrial intermembrane space bridging) CHCHD3 is highly conserved in mammals with human and mouse protein sharing ∼92% sequence similarity.

Publications

Species	Application	Title
human	WB	Nat Commun; Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis; Authors - Ji Geng
mouse	IF	Acta Neuropathol; Mitochondria, ER, and nuclear membrane defects reveal early mechanisms for upper motor neuron vulnerability with respect to TDP-43 pathology.; Authors - Mukesh Gautam
human	WB	Mol Cell; Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria.; Authors - Milena Vukotic
mouse,human	WB	Nat Commun; Mitochondrial membrane proteins and VPS35 orchestrate selective removal of mtDNA; Authors - Ayesha Sen
human	WB	EMBO Rep; Cristae undergo continuous cycles of membrane remodelling in a MICOS-dependent manner.; Authors - Arun Kumar Kondadi
mouse	WB	Cell Rep; Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control.; Authors - Christina Glytsou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mia (Verified Customer) (09-28-2022)

goood！

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse tissue