CHCHD3 Polyclonal antibody

CHCHD3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25625-1-AP

Synonyms

CHCHD3, MIC19, MINOS3



Tested Applications

Positive WB detected inHEK-293 cells, human placenta tissue, A431 cells, mouse liver tissue, NIH/3T3 cells, Jurkat cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:4000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25625-1-AP is CHCHD3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHCHD3 fusion protein Ag22579
Full Name coiled-coil-helix-coiled-coil-helix domain containing 3
Calculated molecular weight 227 aa, 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC014839
Gene symbol CHCHD3
Gene ID (NCBI) 54927
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CHCHD3 antibody 25625-1-APDownload protocol
IHC protocol for CHCHD3 antibody 25625-1-APDownload protocol
IF protocol for CHCHD3 antibody 25625-1-APDownload protocol
IP protocol for CHCHD3 antibody 25625-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Acta Neuropathol

Mitochondria, ER, and nuclear membrane defects reveal early mechanisms for upper motor neuron vulnerability with respect to TDP-43 pathology.

Authors - Mukesh Gautam
humanWB

Mol Cell

Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria.

Authors - Milena Vukotic
humanWB

EMBO Rep

Cristae undergo continuous cycles of membrane remodelling in a MICOS-dependent manner.

Authors - Arun Kumar Kondadi
mouseWB

Cell Rep

Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control.

Authors - Christina Glytsou
humanWB

Sci Rep

Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure.

Authors - Chengli Ding
humanWB

Hum Mol Genet

Loss of CHCHD10-CHCHD2 complexes required for respiration underlies the pathogenicity of a CHCHD10 mutation in ALS.

Authors - Isabella R Straub