CHD3 Polyclonal antibody

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• CHD3 Polyclonal antibody (25335-1-AP)

CHD3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25335-1-AP Copied!

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA
For FC, select conjugated version.

Synonyms

ATP-dependent helicase CHD3, CHD 3, CHD-3, Chromodomain-helicase-DNA-binding protein 3, EC:3.6.4.12

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  WB analysis using 25335-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25335-1-AP (CHD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of HEK-293 using 25335-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25335-1-AP (CHD3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human kidney using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of mouse brain using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25335-1-AP (CHD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25335-1-AP (CHD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25335-1-AP (CHD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25335-1-AP (CHD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: MCF-7 cells, HEK-293 cells, mouse brain tissue
• Positive IHC detected in: mouse brain tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25335-1-AP targets CHD3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: chromodomain helicase DNA binding protein 3
• Calculated Molecular Weight: 227 kDa
• Observed Molecular Weight: 240-250 kDa
• GenBank Accession Number: NM_005852
• Gene Symbol: CHD3
• Gene ID (NCBI): 1107
• RRID: AB_2880033
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q12873
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHD3, also named as Mi2 ALPHA or Zinc finger helicase, is a 2000 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and two chromo domain. CHD3 localizes in the nucleus and cytoplasm. CHD3 is widely expressed. CHD3 is a component of the histone deacetylase NuRD complex, which participates in the remodeling of chromatin by deacetylating histones. CHD3 is required for anchoring centrosomal pericentric in both interphase and mitosis.

Publications

What published studies show

The CHD3 polyclonal antibody (Cat# 25335-1-AP) has 3 citations published in Molecular Cell, Nucleic Acids Research, and PNAS. The cited studies focus on chromatin remodeling via the NuRD complex, epigenome reprogramming, and cancer biology—specifically breast cancer development and prostate cancer immune evasion through the STAT1–MHC-I axis. All citations used Western blot as the application in human samples.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cell; NuRD-enabled CTCF-TET crosstalk orchestrates epigenome reprogramming and genome architecture.; Authors - Wenju Sun
human	WB	Nucleic Acids Res; CARM1 hypermethylates the NuRD chromatin remodeling complex to promote cell cycle gene expression and breast cancer development; Authors - Xue Chen
	WB	Proc Natl Acad Sci U S A; ZNF263-NuRD-mediated repression of STAT1 curtails MHC-I antigen presentation and IFN-γ efficacy in prostate cancer; Authors - Han Guan