• Featured Product
  • KD/KO Validated

CHDH Polyclonal antibody

CHDH Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 17356-1-AP

Synonyms

CDH, CHD, CHDH, Choline dehydrogenase



Tested Applications

Positive WB detected inrat liver tissue, mouse kidney tissue, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17356-1-AP targets CHDH in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHDH fusion protein Ag10614
Full Name choline dehydrogenase
Calculated molecular weight 594 aa, 65 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC034502
Gene symbol CHDH
Gene ID (NCBI) 55349
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHDH(Choline dehydrogenase, mitochondrial) is also named as CDH,CHD and belongs to the GMC oxidoreductase family. CHDH is a member of the glucose-methanol-choline family of oxidoreductases, with each member containing a consensus FAD-binding domain. Many members of this oxidoreductase family are soluble proteins but mammalian CHDH is associated with the inner mitochondrial membrane(Handbook of vitamins,Robert B,2007). The full length protein(65 kDa) has a transit peptide of 29 amino acid and the mature CHDH is appropriately located in the inner mitochondria membrane(PMID:12951056). 17356-1-AP can also recognize the 45 kDa isoform X3 of CHDH.

Protocols

Product Specific Protocols
WB protocol for CHDH antibody 17356-1-APDownload protocol
IHC protocol for CHDH antibody 17356-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

PLoS One

Choline dehydrogenase polymorphism rs12676 is a functional variation and is associated with changes in human sperm cell function.

Authors - Johnson Amy R AR
mouseWB

J Biol Chem

Betaine is accumulated via transient choline dehydrogenase activation during Mouse oocyte meiotic maturation.

Authors - Taylor McClatchie
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hua (Verified Customer) (12-04-2018)

40 ug of mouse liver lysate was loaded onto the gel. The incubation time is over night at 4oC.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse liver
CHDH Antibody Western Blot, validation (1:1000 dilution) in mouse liver (Cat no:17356-1-AP)