Chk1 Monoclonal antibody

Chk1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b


human, mouse, rat







Cat no : 60277-1-Ig


CHEK1, Chk1

Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

60277-1-Ig targets Chk1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Chk1 fusion protein Ag0409
Full Name CHK1 checkpoint homolog (S. pombe)
Calculated Molecular Weight 54 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC004202
Gene Symbol CHEK1
Gene ID (NCBI) 1111
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.


Product Specific Protocols
WB protocol for Chk1 antibody 60277-1-IgDownload protocol
IHC protocol for Chk1 antibody 60277-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Struct Mol Biol

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

Authors - Xinxin Zuo

Cell Death Discov

Comparable radiation sensitivity in p53 wild-type and p53 deficient tumor cells associated with different cell death modalities.

Authors - Ping Li

Acta Biochim Biophys Sin (Shanghai)

The FBXO32/ATR/ATM axis acts as a molecular switch to control the sensitivity of osteosarcoma cells to irradiation through its regulation of EXO1 expression

Authors - Yao Lu

Antiviral Res

Proteomic and phosphoproteomic analysis of responses to enterovirus A71 infection reveals novel targets for antiviral and viral replication

Authors - Dandan Lin


Epiberberine induced p53/p21-dependent G2/M cell cycle arrest and cell apoptosis in gastric cancer cells by activating γ-aminobutyric acid receptor- β3

Authors - Mengmeng Li