Chk1 Monoclonal antibody

• Home
• Products
• Primary Antibodies
• Chk1 Monoclonal antibody (60277-1-Ig)

Chk1 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 60277-1-Ig Copied!

Clone No. 3A3A5

Publications (15)

Download datasheet

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CHEK1, Chk1

• 

  WB analysis using 60277-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60277-1-Ig (Chk1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 60277-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60277-1-Ig (Chk1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 60277-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60277-1-Ig (Chk1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 60277-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60277-1-Ig (Chk1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for Chk1 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60277-1-Ig targets Chk1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0409

  Product name: Recombinant human CHK1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 42-256 aa of BC004202

  Sequence: MKRAVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITI

• Full Name: CHK1 checkpoint homolog (S. pombe)
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC004202
• Gene Symbol: Chk1
• Gene ID (NCBI): 1111
• RRID: AB_2881396
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O14757
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.

Publications

What published studies show

The anti-CHK1 antibody (Cat# 60277-1-Ig) has 15 total citations across journals including Cell Death & Disease, Phytomedicine, Cell Death Discovery, Nature Structural & Molecular Biology, Cell Signaling, iScience, Antiviral Research, Journal of Medical Virology, Medicine (Baltimore), and Inflammation Research. Research fields span cancer biology (gastric, colorectal, prostate, lung, osteosarcoma, GIST), DNA damage response, cell cycle regulation, apoptosis, viral infection, neurodegeneration, cardiac repair, and inflammation.

Automatically generated from the 15 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Death Dis; Chromatin assembly factor 1 subunit A promotes TLS pathway by recruiting E3 ubiquitin ligase RAD18 in cancer cells; Authors - Bing Wen
human	WB	Phytomedicine; The anticancer effects of Aronia berry extract are mediated by Chk1 and p53 in colorectal cancer; Authors - Yoh Asahi
human	WB	Phytomedicine; Epiberberine induced p53/p21-dependent G2/M cell cycle arrest and cell apoptosis in gastric cancer cells by activating γ-aminobutyric acid receptor- β3; Authors - Mengmeng Li
mouse	WB	Cell Death Discov; Tert promotes cardiac regenerative repair after MI through alleviating ROS-induced DNA damage response in cardiomyocyte; Authors - Xiaomin Wei
human	WB	Cell Death Discov; Comparable radiation sensitivity in p53 wild-type and p53 deficient tumor cells associated with different cell death modalities.; Authors - Ping Li
mouse	WB	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo