CHKA-Specific Polyclonal antibody

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  WB analysis of HepG2 using 19537-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 19537-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 19537-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 19537-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 19537-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 19537-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 19537-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 19537-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 19537-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 19537-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 19537-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19537-1-AP (CHKA-Specific Antibody) at dilution of 1:50(under 10x lens).

• 

  IHC staining of human breast cancer using 19537-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19537-1-AP (CHKA-Specific Antibody) at dilution of 1:50(under 40x lens).

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  IHC staining of human liver using 19537-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19537-1-AP (CHKA-Specific Antibody) at dilution of 1:50(under 10x lens).

• 

  IHC staining of human liver using 19537-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19537-1-AP (CHKA-Specific Antibody) at dilution of 1:50(under 40x lens).

• 

  IF Staining of HepG2 using 19537-1-AP

  Immunofluorescent analysis of HepG2 cells, using CHKA antibody 19537-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

CHKA-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 19537-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CHETK alpha, CHK, CHKA, CHKA-Specific, Choline kinase alpha, CK, CKI, EK, Ethanolamine kinase;

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Tested Applications

• Positive WB detected in: HepG2 cells, COLO 320 cells, human testis tissue, mouse colon tissue, mouse testis tissue, Raji cells
• Positive IHC detected in: human breast cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19537-1-AP targets CHKA-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: choline kinase alpha
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: NM_001277
• Gene Symbol: CHKA
• Gene ID (NCBI): 1119
• RRID: AB_10792247
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35790
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Choline kinase alpha, also named as CHK, CKI and CK, belongs to the choline/ethanolamine kinase family. CHKa may have a regulatory role in phosphatidylcholine synthesis. CHKA catalyze the reaction: ATP + choline = ADP + O-phosphocholine. The human enzyme resembled the rat liver enzyme over the entire sequence. It also resembled the yeast enzyme in the carboxy-terminal region, but not in the amino-terminal region(PMID:1618328).And it expresses in human brain tissue but not in human skeletal muscle(PMID:21665002).It has 2 isoforms produced by alternative splicing.The antibody is specific to CHKA.