CHMP7 Polyclonal antibody

• Featured Product
• KD/KO Validated

CHMP7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

12

Reviews (1)

Cat no : 16424-1-AP

Synonyms

Chromatin-modifying protein 7, Chromatin modifying protein 7

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Validation Data Gallery

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  WB analysis using 16424-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16424-1-AP (CHMP7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16424-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16424-1-AP (CHMP7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spinal cord using 16424-1-AP

  mouse spinal cord tissue were subjected to SDS PAGE followed by western blot with 16424-1-AP (CHMP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spinal cord using 16424-1-AP

  rat spinal cord tissue were subjected to SDS PAGE followed by western blot with 16424-1-AP (CHMP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 16424-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16424-1-AP (CHMP7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 16424-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16424-1-AP (CHMP7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 16424-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16424-1-AP (CHMP7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 16424-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CHMP7 antibody (16424-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of A431 using 16424-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CHMP7 antibody (16424-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HEK-293T cells, mouse spinal cord tissue, rat spinal cord tissue, HeLa cells, Jurkat cells
• Positive IHC detected in: human kidney tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 3 publications below

Product Information

16424-1-AP targets CHMP7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHMP7 fusion protein Ag9625
• Full Name: CHMP family, member 7
• Calculated Molecular Weight: 453 aa, 51 kDa
• Observed Molecular Weight: 51-55 kDa
• GenBank Accession Number: BC019110
• Gene Symbol: CHMP7
• Gene ID (NCBI): 91782
• RRID: AB_2079500
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell; TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival; Authors - Pauline Lascaux
human	WB,IF	Nat Cell Biol; Unrestrained ESCRT-III drives micronuclear catastrophe and chromosome fragmentation.; Authors - Marina Vietri ; KD Validated
human	WB	Dev Cell; CC2D1B Coordinates ESCRT-III Activity during the Mitotic Reformation of the Nuclear Envelope.; Authors - Leandro N Ventimiglia ; KD Validated
human	WB	Dev Cell; The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair.; Authors - Samuel S Wallis ; KD Validated
human	WB	Curr Biol; Membrane Binding by CHMP7 Coordinates ESCRT-III-Dependent Nuclear Envelope Reformation.; Authors - Yolanda Olmos ; KD Validated
human	WB,IP	Elife; CDK1 controls CHMP7-dependent nuclear envelope reformation.; Authors - Alberto T Gatta ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Viviana (Verified Customer) (06-01-2022)

Very good to IP endogenous protein from mouse tissue (2 ug for less than 2mg of total protein tested) and also for western blot.

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse embryonic tissue

CHMP7 Antibody Western Blot,Immunoprecipitation validation (1:1000 dilution) in mouse embryonic tissue (Cat no:16424-1-AP)