CHMP7 Polyclonal antibody

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• CHMP7 Polyclonal antibody (16424-1-AP)

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• KD/KO Validated

CHMP7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16424-1-AP

Publications(23)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Chromatin-modifying protein 7, Chromatin modifying protein 7

• 

  WB analysis using 16424-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16424-1-AP (CHMP7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 16424-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16424-1-AP (CHMP7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse spinal cord using 16424-1-AP

  mouse spinal cord tissue were subjected to SDS PAGE followed by western blot with 16424-1-AP (CHMP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat spinal cord using 16424-1-AP

  rat spinal cord tissue were subjected to SDS PAGE followed by western blot with 16424-1-AP (CHMP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 16424-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16424-1-AP (CHMP7 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 16424-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16424-1-AP (CHMP7 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 16424-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16424-1-AP (CHMP7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A431 using 16424-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CHMP7 antibody (16424-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A431 using 16424-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CHMP7 antibody (16424-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, HEK-293T cells, mouse spinal cord tissue, rat spinal cord tissue, HeLa cells, Jurkat cells
• Positive IHC detected in: human kidney tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16424-1-AP targets CHMP7 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9625

  Product name: Recombinant human CHMP7 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 103-453 aa of BC019110

  Sequence: ELQRESDFMASVDSSWISWGVGVFLLKPLKWTLSNMLGDNKVPAEEVLVAVELLKEKAEEVYRLYQNSPLSSHPVVALSELSTLCANSCPDERTFYLVLLQLQKEKRVTVLEQNGEKIVKFARGPRAKVSPVNDVDVGVYQLMQSEQLLSRKVESLSQEAERCKEEARRACRAGKKQLALRSLKAKQRTEKRIEALHAKLDTVQGILDRIYASQTDQMVFNAYQAGVGALKLSMKDVTVEKAESLVDQIQELCDTQDEVSQTLAGGVTNGLDFDSEELEKELDILLQDTTKEPLDLPDNPRNRHFTNSVPNPRISDAELEAELEKLSLSEGGLVPSSKSPKRQLEPTLKPL

• Full Name: CHMP family, member 7
• Calculated Molecular Weight: 453 aa, 51 kDa
• Observed Molecular Weight: 51-55 kDa
• GenBank Accession Number: BC019110
• Gene Symbol: CHMP7
• Gene ID (NCBI): 91782
• RRID: AB_2079500
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WUX9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell; TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival; Authors - Pauline Lascaux
human	WB,IF	Nat Cell Biol; Unrestrained ESCRT-III drives micronuclear catastrophe and chromosome fragmentation.; Authors - Marina Vietri ; KD Validated
human	WB	Dev Cell; CC2D1B Coordinates ESCRT-III Activity during the Mitotic Reformation of the Nuclear Envelope.; Authors - Leandro N Ventimiglia ; KD Validated
	WB	Nat Struct Mol Biol; ESCRT-III assembles around mis-segregated DNA to protect genome stability; Authors - James Glover ; KD Validated
human	WB	Dev Cell; The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair.; Authors - Samuel S Wallis ; KD Validated
human	WB	Curr Biol; Membrane Binding by CHMP7 Coordinates ESCRT-III-Dependent Nuclear Envelope Reformation.; Authors - Yolanda Olmos ; KD Validated

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

Automatically generated from the verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Viviana (Verified Customer) (06-01-2022)

Very good to IP endogenous protein from mouse tissue (2 ug for less than 2mg of total protein tested) and also for western blot.

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse embryonic tissue

CHMP7 Antibody Western Blot,Immunoprecipitation validation (1:1000 dilution) in mouse embryonic tissue (Cat no:16424-1-AP)