CHRNA9 Polyclonal antibody

• Featured Product
• KD/KO Validated

CHRNA9 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26025-1-AP

Synonyms

CHRNA9, HSA243342, NACHR alpha 9, NACHRA9

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Validation Data Gallery

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  WB analysis of mouse skin using 26025-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 26025-1-AP (CHRNA9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 26025-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 26025-1-AP (CHRNA9 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 26025-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 26025-1-AP (CHRNA9 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse skin tissue
• Positive IHC detected in: human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• FC: See 1 publications below

Product Information

The immunogen of 26025-1-AP is CHRNA9 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHRNA9 fusion protein Ag23272
• Full Name: cholinergic receptor, nicotinic, alpha 9
• Calculated Molecular Weight: 479 aa, 55 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC113549
• Gene Symbol: CHRNA9
• Gene ID (NCBI): 55584
• RRID: AB_2880341
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	FC	Mar Drugs; Differential Expression of Nicotine Acetylcholine Receptors Associates with Human Breast Cancer and Mediates Antitumor Activity of αO-Conotoxin GeXIVA.; Authors - Zhihua Sun
human	WB	Mar Drugs; αO-Conotoxin GeXIVA Inhibits the Growth of Breast Cancer Cells via Interaction with α9 Nicotine Acetylcholine Receptors.; Authors - Zhihua Sun ; KO Validated