Tested Applications
| Positive WB detected in | mouse skin tissue |
| Positive IHC detected in | human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26025-1-AP targets CHRNA9 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23272 Product name: Recombinant human CHRNA9 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 324-479 aa of BC113549 Sequence: HFCGAEARPVPHWARVVILKYMSRVLFVYDVGESCLSPHHSRERDHLTKVYSKLPESNLKAARNKDLSRKKDMNKRLKNDLGCQGKNPQEAESYCAQYKVLTRNIEYIAKCLKDHKATSSKGSEWKKVAKVIDRFFMWIFFIMVFVMTILIIARAD Predict reactive species |
| Full Name | cholinergic receptor, nicotinic, alpha 9 |
| Calculated Molecular Weight | 479 aa, 55 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC113549 |
| Gene Symbol | CHRNA9 |
| Gene ID (NCBI) | 55584 |
| RRID | AB_2880341 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UGM1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CHRNA9 (nicotinic α9 subunit of cholinergic receptor) gene is located in zone 4 (4p14) of short arm 1 of human chromosome 4, encoding a ligand-gated divalent cation channel protein, belonging to the nicotinic acetylcholine receptor (nAChR) superfamily. This subunit can be combined with CHRNA10 subunit to form a heteropentamer or a homopentamer, which is mainly distributed in cochlear outer hair cells, dorsal root ganglion, keratinocytes and immune cells.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CHRNA9 antibody 26025-1-AP | Download protocol |
| IHC protocol for CHRNA9 antibody 26025-1-AP | Download protocol |
| WB protocol for CHRNA9 antibody 26025-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CHRNA9 antibody (Cat# 26025-1-AP) has 3 total citations. These appear in Marine Drugs (2 papers) and Archives of Biochem Biophys (1 paper). The research fields span breast cancer biology—specifically the role of α9 nicotinic acetylcholine receptors in tumor growth and antitumor activity of αO-Conotoxin GeXIVA—and colorectal cancer metastasis, where m¹A modification-mediated tRNA shearing suppresses CHRNA9 expression to promote metastatic progression.
| Species | Application | Title |
|---|---|---|
Mar Drugs αO-Conotoxin GeXIVA Inhibits the Growth of Breast Cancer Cells via Interaction with α9 Nicotine Acetylcholine Receptors.
| ||
Mar Drugs Differential Expression of Nicotine Acetylcholine Receptors Associates with Human Breast Cancer and Mediates Antitumor Activity of αO-Conotoxin GeXIVA. | ||
Arch Biochem Biophys tsRNA-10547 generated by m(1)A modification-mediated tRNA shearing promotes colorectal cancer metastasis via suppressing CHRNA9. |
