CHRNA9 Recombinant monoclonal antibody

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• CHRNA9 Recombinant monoclonal antibody (87585-1-RR)

CHRNA9 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87585-1-RR

Clone No.252931C6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

HSA243342, NACHR alpha 9, NACHR alpha-9, NACHRA9, Neuronal acetylcholine receptor subunit alpha-9

• 

  WB analysis using 87585-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87585-1-RR (CHRNA9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 87585-1-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 87585-1-RR (CHRNA9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, mouse skin tissue, rat skin tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87585-1-RR targets CHRNA9 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag23272

  Product name: Recombinant human CHRNA9 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 324-479 aa of BC113549

  Sequence: HFCGAEARPVPHWARVVILKYMSRVLFVYDVGESCLSPHHSRERDHLTKVYSKLPESNLKAARNKDLSRKKDMNKRLKNDLGCQGKNPQEAESYCAQYKVLTRNIEYIAKCLKDHKATSSKGSEWKKVAKVIDRFFMWIFFIMVFVMTILIIARAD

• Full Name: cholinergic receptor, nicotinic, alpha 9
• Calculated Molecular Weight: 479 aa, 55 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC113549
• Gene Symbol: CHRNA9
• Gene ID (NCBI): 55584
• RRID: AB_3745638
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UGM1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHRNA9 (nicotinic α9 subunit of cholinergic receptor) gene is located in zone 4 (4p14) of short arm 1 of human chromosome 4, encoding a ligand-gated divalent cation channel protein, belonging to the nicotinic acetylcholine receptor (nAChR) superfamily. This subunit can be combined with CHRNA10 subunit to form a heteropentamer or a homopentamer, which is mainly distributed in cochlear outer hair cells, dorsal root ganglion, keratinocytes and immune cells.