CHRNB1 Polyclonal antibody

CHRNB1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 11553-1-AP

Synonyms

ACHRB, CHRNB, CHRNB1, CMS1D, CMS2A, SCCMS

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Validation Data Gallery

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  WB analysis of human liver using 11553-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11553-1-AP (CHRNB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 11553-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11553-1-AP (CHRNB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 11553-1-AP

  IP result of anti-CHRNB1 (IP:11553-1-AP, 4ug; Detection:11553-1-AP 1:300) with mouse liver tissue lysate 4000ug.

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  IHC staining of human colon cancer using 11553-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11553-1-AP (CHRNB1 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: human liver tissue, mouse liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below

Product Information

11553-1-AP targets CHRNB1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHRNB1 fusion protein Ag2108
• Full Name: cholinergic receptor, nicotinic, beta 1 (muscle)
• Calculated Molecular Weight: 501 aa, 57 kDa
• Observed Molecular Weight: 50-57 kDa
• GenBank Accession Number: BC023553
• Gene Symbol: CHRNB1
• Gene ID (NCBI): 1140
• RRID: AB_2291854
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acetylcholine receptors (AChRs) are integral membrane proteins that respond to the binding of acetylcholine (ACh), a neurotransmitter synthesized, stored and finally released by cholinergic neurons. After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The muscle acetylcholine receptor is composed of five subunits: two alpha subunits and one each of the beta, delta, and gamma (in immature muscle) or epsilon (in mature muscle) subunits. CHRNB1 gene encodes acetylcholine receptor subunit beta (ACHRB). Mutations in this gene are associated with slow-channel congenital myasthenic syndrome.

Publications

Species	Application	Title
mouse	WB	Aging (Albany NY); A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.; Authors - Xiao Wu
mouse	WB	Aging (Albany NY); Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.; Authors - Dongdong Wang
mouse	WB	Transl Psychiatry; A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota.; Authors - Yinrui Guo
mouse	WB	Genes Immun; Plasticity of the murine spleen T-cell cholinergic receptors and their role in in vitro differentiation of na?ve CD4 T cells toward the Th1, Th2 and Th17 lineages.; Authors - Qian J J
mouse	WB	Biochem Biophys Res Commun; Association of a newly identified lncRNA LNC_000280 with the formation of acetylcholine receptor clusters in vitro.; Authors - Jin Deng
mouse	WB	Mol Ther Nucleic Acids; MiR-142a-3p: A novel ACh receptor transcriptional regulator in association with peripheral nerve injury; Authors - Zhidan Qi