Tested Applications
| Positive WB detected in | mouse heart tissue, rat heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55360-1-AP targets CHRNE in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | cholinergic receptor, nicotinic, epsilon |
| Calculated Molecular Weight | 55 kDa |
| Observed Molecular Weight | 40-50 kDa |
| GenBank Accession Number | NM_000080 |
| Gene Symbol | CHRNE |
| Gene ID (NCBI) | 1145 |
| RRID | AB_3086443 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q04844 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Acetylcholine receptor subunit epsilon is a protein that in humans is encoded by the CHRNE gene. Acetylcholine receptors at mature mammalian neuromuscular junctions are pentameric protein complexes composed of four subunits in the ratio of two alpha subunits to one beta, one epsilon, and one delta subunit. The acetylcholine receptor changes subunit composition shortly after birth when the epsilon subunit replaces the gamma subunit seen in embryonic receptors. Mutations in the epsilon subunit are associated with congenital myasthenic syndrome.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CHRNE antibody 55360-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CHRNE Polyclonal antibody (Cat# 55360-1-AP) has 1 total citation. It appears in Cell and Molecular Life Sciences (IF 6.5), published in 2026. The cited study investigates the miR-206-3p/Cpeb1 axis in delaying acetylcholine receptor degradation and preserving neuromuscular junction stability during denervation-induced muscle atrophy, placing it in the fields of neuromuscular biology and muscle physiology.
| Species | Application | Title |
|---|---|---|
Cell Mol Life Sci The miR-206-3p/Cpeb1 axis delays acetylcholine receptor degradation and preserves neuromuscular junction stability in denervation-induced muscle atrophy. |
