CHST13-Specific Polyclonal antibody

CHST13-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20141-1-AP

Synonyms

CHST13, Chondroitin 4-sulfotransferase 3, Chondroitin 4-O-sulfotransferase 3, C4ST-3, C4ST3

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Validation Data Gallery

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  WB analysis of mouse liver using 20141-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20141-1-AP (CHST13-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 20141-1-AP

  IP result of anti-CHST13-Specific (IP:20141-1-AP, 4ug; Detection:20141-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human kidney using 20141-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20141-1-AP (CHST13-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 20141-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20141-1-AP (CHST13-Specific antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 20141-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20141-1-AP (CHST13-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 20141-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20141-1-AP (CHST13-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 20141-1-AP

  Immunofluorescent analysis of Hela cells, using CHST13 antibody 20141-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human kidney tissue, human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20141-1-AP targets CHST13-Specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: carbohydrate (chondroitin 4) sulfotransferase 13
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: NM_152889
• Gene Symbol: CHST13
• Gene ID (NCBI): 166012
• RRID: AB_10667458
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHST13, also named as C4ST3, belongs to the sulfotransferase 2 family. CHST13 catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. CHST13 transfers sulfate to the C4 hydroxyl of beta1,4-linked GalNAc that is substituted with a beta-linked glucuronic acid at the C-3 hydroxyl. No activity toward dermatan. The antibody is specific to CHST13.