CHST15 Polyclonal antibody

• Featured Product
• KD/KO Validated

CHST15 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 14298-1-AP

Synonyms

BRAG, CHST15, GALNAC4S 6ST, GALNAC4S6ST, hBRAG, KIAA0598, RP11 47G11.1

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Validation Data Gallery

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  WB analysis using 14298-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14298-1-AP (CHST15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 14298-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14298-1-AP (CHST15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 14298-1-AP

  IP result of anti-CHST15 (IP:14298-1-AP, 4ug; Detection:14298-1-AP 1:500) with Raji cells lysate 3200ug.

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse spleen tissue, Raji cells, U-937 cells, mouse heart tissue, rat heart tissue
• Positive IP detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

14298-1-AP targets CHST15 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHST15 fusion protein Ag5679
• Full Name: carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 49 kDa, 69 kDa
• GenBank Accession Number: BC050540
• Gene Symbol: CHST15
• Gene ID (NCBI): 51363
• RRID: AB_2081161
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Carbohydrate sulfotransferase 15, also known as CHST15, BRAG, GALNAC4S6ST and KIAA0598, is a single-pass type II membrane protein which belongs to the sulfotransferase 1 family. CHST15 is a sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO4) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. CHST15 may also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and / or regulation of B-cell-specific RAG expression. CHST15 is expressed in B-cell-enriched tissues but not in fetal or adult thymus. It is expressed in fetal and adult spleen, lymph node, tonsil, bone marrow and peripheral leukocytes. It can be detected as 49 kDa and 69 kDa.

Publications

Species	Application	Title
mouse	WB	Elife; Chondroitin sulfate proteoglycan 4,6 sulfation regulates sympathetic nerve regeneration after myocardial infarction.; Authors - Matthew R Blake ; KD Validated
mouse	WB	Am J Physiol Heart Circ Physiol; Hypertension-induced heart failure disrupts cardiac sympathetic innervation; Authors - Arianna Scalco
human	IHC	Osteoarthritis Cartilage; Changes in the metabolism of chondroitin sulfate glycosaminoglycans in articular cartilage from patients with Kashin-Beck Disease.; Authors - Mingxiu Luo
human	IF	Ann Transl Med; Transcriptome analysis of tumor-derived mesenchymal progenitor cells shows that CHST15 is a fibrosis regulator of retroperitoneal liposarcoma.; Authors - Yang Sun
mouse	WB	Physiol Rep; Loss of chondroitin sulfate proteoglycan sulfation allows delayed sympathetic reinnervation after cardiac ischemia-reperfusion; Authors - Matthew R Blake